A multiplex reaction can contain up to eight primers and four probes (to produce four amplicons), so it is good practice to minimize conditions that result in primer dimer formation or other unfavorable interactions. Make sure that amplicons do not overlap. If the ...
Define "primer self-complementarity" and explain what is the phenomena of primer loop and primer dimer in PCR. What does PCR stand for and what is its general purpose? (a) How does PCR work? (b) Why is it useful? How does PCR work and why is it usefu...
Multiplexing approaches for LAMP tests are less developed than for PCR. The larger number of primers per target in LAMP test increases the likelihood of primer-primer interactions for multiplexed target sets. The product of LAMP assay is a series of concatemers of the target region, giving rise...
Why do RNA polymerases not require a primer while DNA polymerase need it? What are the steps in the PCR cycle and what is the purpose for each? Why must scientists use PCR when they are studying a single gene found in the human genome? a) Scientists need many copies of the gene ...
aBoth the powers of the federal government and the state governments are further limited by certain constitutional guarantees of civil liberties (such as “The Bill of Rights”). It seems that there is democracy implied in the system of checks and balances. It is true that this system can re...
Here's a primer on buprenorphine, how it works, and what lifting prescription regulations means for patients and their physicians. What is buprenorphine? Buprenorphine, sold under such brand names as Sublocade and Suboxone, is an opioid. It can be prescribed to treat pain, but is also effectiv...
a.To control the efficiency of the qPCR reaction b.To quantify the RNA in the samples c.To normalize the expression of target genes d.To minimize primer dimers e.To ensure probe is working for the target gene There are 2...
[77]. During conjugation, TraC1 is co-transferred with RP4 into the recipient cell, where it acts to generate RNA primers to initiate complementary strand synthesis of the T-strand [79]. Since the N-terminal portion of TraC1 is necessary for the conjugative transfer of TraC1, ArdC was ...
Basu and Tumban Virology Journal (2016) 13:165 DOI 10.1186/s12985-016-0623-2 REVIEW Open Access Zika Virus on a Spreading Spree: what we now know that was unknown in the 1950's Rupsa Basu and Ebenezer Tumban* Abstract Zika virus (ZIKV) is a mosquito-borne flavivirus that is transmitted...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a potentially life-threatening disease, defined as Coronavirus Disease 19 (COVID-19). This pathological condition is generally characterized by the presence of several signs and symptoms. The most common ones include cough, sore thr...