The hole-punch method is the only suitable diffusion technique for aqueous extracts, because interference by particulate matter is much less than with other types of reservoirs. To ensure that the sample does not leak under the agar layer, fixed agar is left on the bottom of the hole (Cole,...
IVT mRNA is produced from a linear DNA template containing a bacteriophage promotor, the optimized UTR’s and the codon optimized sequence by using a RNA polymerase (T7, T3 or SP6) and a mix of the different nucleosides [7]. The cap structure and the poly A tail can be incorporated duri...
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It is obvious that 1 set of criteria is much more usable in clinical practice than 2 different sets. The presence of erosions at the first visit is an important predictor of persistent arthritis at 2 years and therefore has been incorporated in the model. When erosions are present at the ...
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Infection with hepatitis B virus (HBV) may lead to acute or chronic hepatitis. HBV infections were previously much more frequent but there are still 240 million chronic HBV carriers today and ca. 620,000 die per year from the late sequelae liver cirrhosi
First of all, with mRNA expression of the encoded Ab is detectable for a few days in contrast to a single protein shot with the protein-format. Secondly, with mRNA it is easier to deliver intrabodies as mRNA is transfected in the cells while it is much harder to get the protein-format...
was about 1 h or longer and resulted in a 5-log reduction of M13 phage titer [95]. Gel electrophoresis results indicated that laser irradiation does not change the structure of single-stranded DNA but leads to the breaking of hydrogen/hydrophobic bonds or the separation of weak protein ...
The hybrid filter was expected to be more similar to the performance of the MWNT filter, since the nanotubes were made of 83% MWNT and only 17% SWNT, and SWNT alone had a much lower efficiency. However, the SWNT–MWNT dual filter performed better than the 100% MWNT filter, and is ...