1 × LightCycler FastStart mix, 3 mM MgCl2; 0.5 units of heat-labile uracil-N-glycosylase [30]; 5 μl the internal control at 400 copies/μl; 400 nM of each adenovirus primer (AdV2F, AdV2R, AdV4F, AdV4R)
First, we characterized cellular changes in the hippocampi of mice subjected to moderate TBI and how this correlates to changes in hippocampus-dependent cognition. For this, we compared sham craniotomized mice (Control) and mice subjected to unilateral controlled cortical impact (TBI). Fifteen days ...