1 × LightCycler FastStart mix, 3 mM MgCl2; 0.5 units of heat-labile uracil-N-glycosylase [30]; 5 μl the internal control at 400 copies/μl; 400 nM of each adenovirus primer (AdV2F, AdV2R, AdV4F, AdV4R)
Nestin-GFP mice in which GFP is expressed in individual cells of the AHN niche under the control of the neuroepithelial stem cell protein (Nestin) promoter25,26has been used in single-cell RNA sequencing (scRNA-seq) studies of the AHN niche to sort NSCs, neural progenitor and other cell p...
The initial virus titer for each experiment was ~2 × 104 PFU/mL. All experiments were conducted in a biosafety cabinet at room temperature. The surviving TV was quantified using a standard plaque assay. The negative control was the virus suspension deposited on the drywall and covered with a...
Animals engage in various symbioses. However, these interactions are not always beneficial for the host; they can also incur costs under certain circumstances. The bacterial symbiont supports, on the one hand, the cuticle formation of the sawtoothed grain beetleOryzaephilus surinamensis,which is extr...