In addition, 268 whole blood samples from routine patient requests for B27-associated disease typing were tested in parallel with HLA-B typing using the standard complement-dependent microlymphocytoxicity test. The detailed specificity of the three monoclonal antibodies was established and the products...
Enhanced Chemi- luminescence kit (Pierce, Rockford, IL, USA) and the imaging equipment were used to detect the HRP-labeled proteins on membrane (UVP ChemStudio PLUS Touch, Analytik Jena US LLC, Cambridge, UK). Band intensities were quantified from the digital image by densitometry using ImageJ...