生国杂志2002年6月第l5卷第3期ChinJBloodTransfusion,June,2002,Vol15,No.3 流式细胞仪检测HLA—B27抗原初探 许先国洪小珍何吉金蕾傅启华严力行(浙江省血液中心,浙江杭州310020) - l65 摘要:目的建立一种快速,准确的HLA—B17检测技术.方法利用流武细胞仪(FCM)检测 HLA—B27抗原,并 ...
Whole blood (5 mL) collected in a green-top (heparin) or a yellow-top (acid-citrate-dextrose [ACD]) tube. Normal findings (Method: Flow cytometry) Negative (indicating absence of the antigen). Description The human leukocyte antigens (HLAs) are gene products of the major histocompatibilit...
进行同意后筛查,排除HepBSAg、HepBcAb、HepCAb、HIV或HCG检测呈阳性的人。 Randomisation and blinding After final eligibility testing, blood was sent for determination of IgG HLA Ab status (see below and Supplementary text), an...
YANG Yu-ting,ZHOU Fen,HUA Yan-jun,et al.Expression of CD4+T Cells in Peripheral Blood of Patients with Pulmonary Tuberculosis and the Assessment of Related Factors by Logistic Regression[J].Journal of Modern Laboratory Medicine,2016,31(01):90.[doi:10.3969/j.issn.1671-7414.2016.02.027][2]李...
Applications Tested: This BB7.1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final...
The distribution of B* 27 alleles was determined in a random population of 4020 local blood donors and the use of PCR- SSP B* 27 typing in our routine flow cytometry- based HLA- B27/ B2708 typing strategy is described.Downing, J
随着记忆性 B 细胞研究的进一步深入, 荷 兰莱顿医学院免疫血液及输血科建立了一种基 于 ELISPOT 的定量检测供体特异性记忆 B 细胞的方法[27]. 该方法主要通过植物血凝素 (phytohaemagglutinin,PHA)刺激供体外周血单个 核细胞(peripheral blood mononuclear cell,PBMC), 使 PBMC 持续分化, 细胞中的 HLA 抗原含量...
Applications Tested: This BB7.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in...
blood cells. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. ...
In addition, 268 whole blood samples from routine patient requests for B27-associated disease typing were tested in parallel with HLA-B typing using the standard complement-dependent microlymphocytoxicity test. The detailed specificity of the three monoclonal antibodies was established and the products...