Biological Activity: Immobilized Anti-NY-ESO-1(HLA-A*02:01) Antibody,hFc Tag at 1 ug/ml (100 μl/Well)on the plate.Dose response curve for Biotinylated Human NY-ESO-1(HLA-A*02:01),His Tag with the EC50 of 16.5ng/ml determined by ELISA. 研究背景:嵌合HLA-A*0201 WT-1 复合物属...
(D) The percentage of activated CD8+ T cells derived as described in (B) that could secrete IFN-γ (FITC-IFN-γ -stained) was quantified by ICS using APC-CD8 antibody staining. Table 3. Concentration of cytokines in supernatant of CD8+ T cell cultures derived from HLA-A∗02:01 and ...
utilizing an anti-human CD3 monoclonal antibody covered by a solid phase and an IL-2 to stimulate the target CTL to grow; adding an autologous PBMC (Peripheral Blood Mononuclear Cell) radiated by gamma rays to enhance the activation of the target CTL; adding rhIL-15 to cultivate and reproduce...
(Ficoll)densitygradientcentrifugation. separated individualsPBMCwere TheHLA-A+0201 identifieddirect positive by FITC—HLA—A木0201monoclonal immunofluorescence antibody; assayusing vitro testlactate (3).In cytotoxicity assay:The HLA—A木0201 individualsPBMCwerestimulatedandculturedinvitro positive T2cellswere...
The HLA-A2 restriction of CD8+ T-cell-mediated lysis was tested at an effector cell to target cell ratio of 30 : 1 in the presence of the anti-HLA-A2 monoclonal antibody derived from the MA2.1 hybridoma (American Type Culture Collection) at a final concentration of 1 μg ml...
Despite several decades of research, there are no effective and safe DV vaccines. Previous studies have shown that preexisting DV non-neutralizing antibodies can enhance secondary heterologous DV serotype infectionsviaantibody-dependent enhancement (ADE). ADE may be the mechanism for development of DHF...
Simultaneous immunization of both CMV proteins, using DNA vaccine priming followed by rAAV boost, induced antibody (Ab) response, CD8 lymphocytes with cytotoxic function, and detectible binding of the cognate peptide epitopes for human HLA A*0201 restriction using tetramer technology....
The refolded and biotinylated products were detected by Western blot and ELISA with monoclonal antibody BB7.2 that recognized the natural conformations of HLA-A2 and streptavidin. The results showed that the refolded complex was composed of HLA-A*0201-BSP aggregate, HLA-A*0201-PR1 complex and ...
stimulating the growth of the target CTL by using solid-phase-coated anti-human CD3 monoclonal antibody and IL-2; activating the autologous PBMC enhancement target CTL which is radiated under gamma rays; and adding rhIL-15, carrying out culture and amplification, collecting and carrying out identif...
, Selection of tumor antigens as targets for immune attack using immunohistochemistry: II. Blood group-related antigens . Int. J. Cancer , 73 , 50 – 56 ( 1997 ). TODO: clickthrough URL MedlineCorps, A.N. , Sowter, H.M. , and Smith, S.K. , Hepatocyte growth factor stimul...