We also found a normal human pancreatic ductal epithelial cell line, HPDE, to be HLA-A0201 positive. Our findings were verified with two different sequence-based typing methods, antibody staining followed by flow cytometry analysis, and functional analysis using an HLA-A0201-restricted peptide-...
At the same time, the incubated cells were collected and stained with human CD8 antibody. After breaking the cell membrane with FIX&PERM antibody cell permeabilization reagents (Invitrogen, GAS003), the cells were stained with human IFN-γ antibody. Intracellular cytokine staining (ICS) and CBA ...
I类抗原:HLA-A、B、C位点 II类抗原:HLA-DR、DP、DQ位点 III类抗原:补体等 HLA检测分辨率: 低分辨率 A*02 高分辨率 A*0201 HLA抗体 群体反应性抗体(Panel reactive antibody,PRA)---移植受者体内所含有的抗HLA抗体的水平。 HLA抗体是由于HLA不相合,而...
HLA, 英文全称Human Lecucyte Antigen，中文名称，人类白细胞抗原。所以，HLA是一类抗原，存在于白细胞表面。HLA对应的抗体叫HLA抗体，即HLA Antibody，HLA抗原和HLA抗体是会发生免疫反应的，而且会引起急性的排斥反应，即GVHD，几分钟内人就会死亡。故而，在进行骨髓移植、器官移植（尤其是肾移植）的时候...
HLA-A*0201 NY-ESO-1 Protein, Human (Biotinylated, HEK293, His-Avi) ,分子量为 51-56 kDa。 Synonyms: MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1 Species: Human Source: HEK293 Biological Activity: Immobilized Anti-NY-ESO-1(HLA-A*02:01) Antibody,...
A—A*0201限制性CTL表位的预测和筛选 th 从SwissProt蛋白数据库获得EBV i、NetCTL、 LMP2全长氨皋酸序列,以Syfpei HLA—Bind、Rankpep等四种在线乍物信息软件预NEBVLMP2中的HI,A-A*0201限制性 CTL表位,综合分析筛选出EBVLMP2中可能存在的CTL表位肽。 2.表位肽的合成 西安华辰牛物科技有限公司采用标准固相肽...
R.V. Lloyd, T.L. Johnson, M. Blaivas, J.C. Sisson, B.S. Wilson. Detection of HLA-DR antigens in paraffin embedded thyroid epithelial cells with a monoclonal antibody, Am J Pathol, 120: 106–111 (1985). PubMedCASGoogle Scholar ...
The HLA-A2 restriction of CD8+ T-cell-mediated lysis was tested at an effector cell to target cell ratio of 30 : 1 in the presence of the anti-HLA-A2 monoclonal antibody derived from the MA2.1 hybridoma (American Type Culture Collection) at a final concentration of 1 μg ml...
The primary and secondary antibodies were stripped with retrieval solution for 20 min before repeating the process with the second primary antibody (position 2) starting with a new application of 3% H2O2. Antibodies included CD8 (clone 144B; Dako; opal fluor 520, concentration 0.2 microgram/mL...
Thermo-life MA518182 ANTIBODY FOR CHIKV (J3 1F) ￥3,519.00 1F) Thermo-life MA518187 CHLAMYDIA TRACHOMATIS LPS ANTI BODY (H85A) ￥3,519.00 BODY (H85A) Thermo-life MA518197 E. COLI O157 ANTIBODY (A19Z) EA ￥3,519.00 EA Thermo-life MA518198 E. COLI O157 ANTIBODY (L65G) EA ￥3...