In this method, magnetic beads are used to isolate B lymphocytes for HLA class II typing from blood or spleen. The remaining leucocytes can be used for HLA class I typing. The lymphocytes are then added to Terasaki plates that hold individual wells containing various specific antibodies. If the...
P251–HLA class1 and class 2 DNA typing of cadaveric cornea donorsp251 - hla class1 and class 2 dna typing of cadaveric cornea donorsdoi:10.1016/0198-8859(96)84946-9N SudraudPY RobertP BertinJP AdenisM CognéM DrouetHuman Immunology...
2HLA分型技术 血清学水平 基因水平 SSP (sequencespecificprimer)SSO (sequencespecificoligonucleotide)SBT (sequence-basedtyping)HLA测序分型（SBT）的PCR策略 HLA–A、C HLA–B HLA测序分型（SBT）的测序策略 HLAClassI(A,BandC)SBT:Exon2 Exon3 Exon4 HLAClassII(DRB1,DQA1,DQB1,DPA1,andDPB1)SBT:Exon2 ...
To determine the impact of high-resolution (HR) HLA typing with outcomes after UCBT, DNAs of 122 pairs were analysed for HLA class I and class II mismatches (MM) based on HR typing. For HLA-A, -B on low-resolution typing and -DRB1 on HR typing, the following MM situation resulted:...
Rapid, highly accurate and cost-effective open-source simultaneous complete HLA typing and phasing of class I and II alleles using nanopore sequencing doi:https://doi.org/10.1111/tan.13926 阅读链接:https://onlinelibrary.wiley.com/doi/10.1111/tan.13926 ...
Cutaneous NVP HSR associates with HLA-C alleles having similar peptide binding properties and F pocket structure asHLA-C*04:01 Four digit HLA typing was available for 151 cases and 413 controls. In single allele logistic regression analysesHLA-C*04:01was the only allele for which a consistent...
HLA typing using RNA-Seq data The tool seq2HLA v2.2 (https://github.com/TRON-Bioinformatics/seq2HLA) was used to derive HLA class I (classical and non-classical) and class II types from the RNA-Seq data. Seq2HLA v2.2 previously determined 4-digit resolution HLA types with high accuracy...
We report the development of a general methodology to genotype HLA class I and class II loci. A Whole Genome Amplification (WGA) step was used as a sample sparing methodology. HLA typing data could be obtained with as few as 300 cells, underlining the usefulness of the methodology for studie...
HLA基因位点全相合和1～2个基因位点不合非亲缘异基因骨髓移植的临床疗效比较,酶切位点,酶切位点保护碱基,地支相合,转录起始位点,八字相合,分位点,多克隆位点,糖基化位点,bamhi酶切位点 文档格式: .pdf 文档大小: 773.15K 文档页数: 31页 顶/踩数: ...
The genes that encode the human leukocyte antigen (HLA) class I and II molecules are highly polymorphic and located in the major histocompatibility complex (MHC) region, where there is a high density of immune-related genes. Numerous studies have identif