HLA DNA typingprincipal component analysisSeven HLA class I and class II loci (HLA-A, B, C, DRB1, DQA1, DPA1 and DPB1) were typed at the DNA level in two populations of the Iberian Peninsula (100 Basque and 88 Catalan individuals) in order to unravel their genetic relationship and to...
Molecular typing of HLA class I and class II antigens in Indian kala-azar patients. Trop Med Int Health 1997;2(5):468-71.Singh, N., Shyam, S., Fionnuala, W., Martin, D., Curran Anill, R. et al. (1997). Molecular typing of HLA class I and class II antigens in Indian Kala...
In this method, magnetic beads are used to isolate B lymphocytes for HLA class II typing from blood or spleen. The remaining leucocytes can be used for HLA class I typing. The lymphocytes are then added to Terasaki plates that hold individual wells containing various specific antibodies. If the...
To determine the impact of high-resolution (HR) HLA typing with outcomes after UCBT, DNAs of 122 pairs were analysed for HLA class I and class II mismatches (MM) based on HR typing. For HLA-A, -B on low-resolution typing and -DRB1 on HR typing, the following MM situation resulted:...
2HLA分型技术 血清学水平 基因水平 SSP (sequencespecificprimer)SSO (sequencespecificoligonucleotide)SBT (sequence-basedtyping)HLA测序分型(SBT)的PCR策略 HLA–A、C HLA–B HLA测序分型(SBT)的测序策略 HLAClassI(A,BandC)SBT:Exon2 Exon3 Exon4 HLAClassII(DRB1,DQA1,DQB1,DPA1,andDPB1)SBT:Exon2 ...
In this study, polymerase chain reaction-sequence-specific oligonucleotide prode (SSOP) typing results for the human leukocyte antigen (HLA) class I (A, B, and C) and class II (DRB1, DQA1, DQB1, and DPB1) loci in 264 individuals of the Han ethnic group from the Canton region of sout...
ClassII(HLA-DR)PolymorphismintheMHCaffectspeptideantigenbindingAllelicvariantsmaydifferby20aminoacids hyf523@yahoo.com Slide2/54 Example:IfMHCXwastheonlytypeofMHCmolecule PathogenthatevadesMHCX MHCXX Survivalofindividualthreatened Populationthreatenedwithextinction hyf523@yahoo.com Slide7/54 Example:Ifeach...
Rapid, highly accurate and cost-effective open-source simultaneous complete HLA typing and phasing of class I and II alleles using nanopore sequencing doi:https://doi.org/10.1111/tan.13926 阅读链接:https://onlinelibrary.wiley.com/doi/10.1111/tan.13926 ...
Many new HLA alleles have been identified in recent years due to the advances in molecular typing technology, but the serological reactivity of a majority of these alleles has not been determined. Various algorithms have been developed that aim to address this problem, and improve the accuracy of...
HLA class I DNA typing of 215 "HLA-A, -B, -DR zero mismatched" kidney transplants DNA typing for HLA class II improves the typing quality and this was shown previously to be relevant for kidney graft survival. In this project we addresse... J Mytilineos,M Lempert,D Middleton,......