HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a ...
目前实验室HLA分型大多为基因分型方法,主要包括低分辨水平的PCR-序列特异性引物基因扩增法(sequence specific primer, SSP)、中分辨水平的PCR-序列特异性寡核苷酸探针法(sequence-specific oligonucleotide, SSO)、基因芯片和高分辨水平的PCR-直接测序分型(sequence-based typing, SBT...
HLA Typing PCR-SSP检测HLA-B27 一、实验目的 掌握PCR-SSP的原理，了解HLA基因分型的基本方法。二、实验原理 编码HLA的基因具有高度的多态性，每一个基因座位上有众多的复等位基因，而每一个等位基因都有其各自的DNA序列，因此可用相应的序列特异性引物（sequencespecificprimers，SSP）进行扩增。通过控制PCR反应条件...
HLA-DRB AND -DBQ TYPING BY PCR AMPLIFICATION USING SEQUENCE-SPECIFIC PRIMERS (PCR-SSP): ASSESSMENT AFTER 1 YEAR OF ROUTINE USE BY THREE LABORATORIES Using sequence-specific amplifications, a practical and fast technique for DRB and DQB typing has been developed. The primers are chosen in ...
At present, our company is mainly through the technology of PCR- sequence specific primers (squence specific, primer, PCR-SSP) and PCR- direct sequence analysis of genotyping technology (sequence based genotyping, PCR-SBT) two kinds of methods to judge the degree of consistency for both sides,...
HLA-DR Typing by Polymerase Chain Reaction with Sequence- Specific Primers Compared to Serological typing Adib, M.; Yaran, M.; Rezaie, A.; et al.: HLA-DR Typing by Polymerase Chain Reaction with Sequence- specific Primers Compared to Serological typing... M Adib,M Yaran,A Rezaie,... ...
HLA-typing is determined through certain antigens in the HLA system. The classical transplantation antigens are HLA-A, -B, -C, -DR, -DQ and -DP. By amplifying the DNA with sequence specific primers in the Polymerase Chain Reaction (PCR), the amplicons can be detected and alleles present...
(polymerase chain reaction-sequence specific oligonucleotide probe,PCR-SSO)和基于DNA序列的分型技术 (sequencing-based typing,SBT).2017~2020年采 用的分型技术类别详见表1.从表中可以看出,PCR- SSP仍然是我国大多数HLA低分辨实验室采用的分型 技术,但PCR-SSO分型技术显著增加,DNA测序技 术占比较小增长率也...
HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens, 39 (5) (1992), pp. 225-235 CrossrefView in ScopusGoogle Scholar...
who were tested positive for HLA-A*02 alleles by PCR with sequence-specific primers (PCR-SSP). Genotyping of the alleles was performed using PCR-sequence-based typing (PCR-SBT).ResultsSix different HLA-A∗02 alleles (A∗020101, A∗0203, A∗0205, A∗0206, A∗0207 and A∗021...