THE polymorph of interest can be detected by PROTOCOLS AND PROPER CONTROL.POULETTY, PHILIPPECHUN, PETERCHANG, CHIN, HAI
Highly polymorphic human leukocyte antigen (HLA) genes are responsible for fine-tuning the adaptive immune system. High-resolution HLA typing is important for the treatment of autoimmune and infectious diseases. Additionally, it is routinely performed for identifying matched donors in transplantation medici...
and nucleic acids obtained by eliminating or adding several bases to the terminals thereof, useful for a method for performing the typing of HLA(Human Leukocyte Antigen)-DQA1 allele and used for the judgment of the compatibility of a donor with a recipient in an organ transplant, an analysis ...
Rapid DNA typing class II antigens using the polymerase chain reaction and reverse dot blot hybridization. HLA-DRtypingReversedot-blothybridizationSequence-specificoligonucleotidePolymerasechainreactionWe established a rapid and simple method of HLA-DR genotyping, and ... R Buyse - 《Tissue Antigens》...
但由于需时较长,其在死亡捐献供者肾移植配型中应用受限,主要用于活体供肾配型及ML的评估以指导术后免疫抑制剂的使用[12]。第三代DNA测序技术检测用时更短、检测结果精度更高,4 h即可实现HLA高分辨率分型,并可精确至第3区域(High reso...
图3. HLAtyping Panel双平台捕获结果 04 Illumina平台HLA分型表现 我们首先使用 HLA-HD、Athlates 和 OptiType 对 HLA 的 I 型和 II 型共计 12 个标准品分析,具体结果见表 3。HLA-HD 和 Athlates 比 OptiType 的 HLA I 型分型更加准确,两者仅在 A 基因的 24 个 allele 上错误分型 1 个位点,而 OptiT...
Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liqu...
参考文献 [1] Duke JL,Lind C,Mackiewicz K,et al. Determining performance characteristics of an NGS-based HLA typing method for clinical applications[J]. HLA,2016,87(3),141-152. [2] Wang C,Krishnakumar S,Wilhelmy J,et al. High-throughput, high-fidelity HLA genotyping with deep sequencing...
Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. ...
9Citations 5Altmetric Metrics Abstract Reduction of adverse drug reaction (ADR) incidence through screening of predisposing human leucocyte antigen (HLA) alleles is a promising approach for many widely used drugs. However, application of these associations has been limited by the cost burden of HLA ...