Piotr, ZąbekTomasz, DydaGrzegorz P., StańczakMagdalena, MarczyńskaEwa, Firląg-BurkackaJanusz J., Stańczak. (2009) Genetic detection of HLA-B*5701 allele for prediction of Abacavir hypersensitivity among HIV-positive patients in Polish population. HIV & AIDS Review 8 , 13-16 /...
induce reactivity in HLA-B*57:01 1 individuals who were not systemically exposed to abacavir? The primary end point was the proportion of positive skin patch test results with abacavir in HLA-B*57:01 1 individuals who had never been exposed to abacavir. The secondary end point was the ...
HIA—B*5701screen— ingwasperformedusingmultiplexedsequencespecificprimersPCR(SSP-PCR)method.Theresultswerereadbyagarose gelelectrophoresis.withadditionalDNAsequencingforIDUswhoseagarosegeleleetrophoresisfindingswerepositive.Re— sulIs Ofthe1043HIVpositiveIDUs5carriedHLA_B ...
Positive Control DNA HLA B*5701 and human DNA (C+)colorless clear liquid0.2Negative Control (C–)*colorless clear liquid0.5* must be used in the isolation procedure as Negative Control of Extraction.2 tubes 2 tubes 2 tubes 1 tube 4 tubes...
Among people with human immunodeficiency virus (HIV) infection, a version of HLA-B designated HLA-B*5701 is associated with an extreme sensitivity toabacavir. This drug is a treatment for HIV-1 that slows the spread of the virus in the body. People with abacavir hypersensitivity often develop...
We also utilized our recently established in vitro T-cell priming assay that recapitulates key elements of events that occur in vivo during elicitation of an immunological drug reaction by combining naive CD3+ T-cells from 3 HLA-B*5701 positive volunteers with flucloxacillin and dendritic cells in...
Prevalence of abacavir-associated hypersensitivity syndrome and HLA-B*5701 allele in a Portuguese HIV-positive populationPrevalence of abacavir-associated hypersensitivity syndrome and HLA-B*5701 allele in a Portuguese HIV-positive populationdoi:10.1016/j.pbj.2016.12.004Abacavir...
the predicted binding orientation of the positive analogs can be well accommodated in the pocket with similar binding behavior as abacavir, whereas the negative analogs all presented unfavorable clashes, suggesting disrupted binding or conformation changes within the HLA-B*57:01 peptide-binding groove. ...
Within the entire abacavir-exposed cohort (n=200), presence of HLA-B *5701, HLA-DR7, and HLA-DQ3 had a positive predictive value for hypersensitivity of 100%, and a negative predictive value of 97%. Genetic susceptibility to abacavir hypersensitivity is carried on the 57·1 ancestral ...
In our study, we have determined the prevalence of HLAB* 5701 in the black HIV positive population in Kenya using a cross sectional epidemiological survey. We recruited 1004 patients from three HIV centers: the Aga Khan University Hospital Nairobi, Mbagathi Hospital and Machakos Hospital in ...