根据杂交信号的有无和强度来确定HLA型别。(4)基于测序的分型方法：SBT（Sequencing-Based Typing）是用两侧引物对HLA基因多态性的区域进行PCR扩增，然后对扩增产物进行Sanger测序，获得HLA等位基因序列从而进行分型。SBT技术是目前HLA基因分型的金标准，具有分辨率高、准确性高以及特异性强的特点，但其针对HLA-I类基因...
HLA-B*5701 typing: evaluation of an allele-specific polymerase chain melting assay. Tissue Antigens. 2007; 70 (1):58–61.Hammond E, Mamotte C, Nolan D, Mallal S. HLA-B*5701 typing: evaluation of an allele- specific polymerase chain reaction melting assay. Tissue Antigens 2007;70(1):...
根据杂交信号的有无和强度来确定HLA型别。 (4)基于测序的分型方法:SBT(Sequencing-Based Typing)是用两侧引物对HLA基因多态性的区域进行PCR扩增,然后对扩增产物进行Sanger测序,获得HLA等位基因序列从而进行分型。SBT技术是目前HLA基因分型的金标准,具有分辨率高、准确性高以及特异性强的特点,但其针对HLA-I类基因的第...
根据杂交信号的有无和强度来确定HLA型别。 (4)基于测序的分型方法:SBT(Sequencing-Based Typing)是用两侧引物对HLA基因多态性的区域进行PCR扩增,然后对扩增产物进行Sanger测序,获得HLA等位基因序列从而进行分型。SBT技术是目前HLA基因分型的金标准,具有分辨率高、准确性高以及特异性强的特点,但其针对HLA-I类基因的第...
预测药物不良反应:某些药物的不良反应与患者的HLA基因型有关。例如,药物阿巴卡韦用于治疗艾滋病时,携带HLA-B*5701基因型的患者发生严重皮肤过敏反应的风险较高。通过检测患者的HLA基因型,可以提前预测其对特定药物的不良反应风险,从而为临床合理用药提供依据,避免严重药物不良反应的发生。
abacavir. Typing the patient's HLA-B*5701 gene before prescribing can reduce this hypersensitivity reaction. Currently, the FDA's black box warning on the abacavir drug label recommends that patients undergo HLA-B*5701 gene testing before using abacavir to reduce the risk of hypersensitivity ...
Objectives: HLA-B*5701 strongly and specifically predicts susceptibility to abacavir hypersensitivity (ABC HSR), however implementation of routine genetic screening into clinical practice requires that testing be both practical and accurate. We assessed the proficiency of HLA-B*5701 typing among numerous ...
HLA分型检测服务HLA typing 博奥生物HLA分型检测实验室采用SBT方法,可进行HLA-A、B、C、DRB1、DQB1高分辨分型服务,高精确、高效率、低价格。广泛用于骨髓库、脐血库等应用。服务类别: 测序/合成 总访问: 8150 最后更新: 2015-6-1 半年访问: 33 参考报价: 咨询查价 立即询价 电话咨询 ...
Despite multiple challenges, molecular docking presents a promising toolkit for investigating drug-HLA interactions and understanding IDR mechanisms, with significant implications for preemptive HLA typing and safer drug development. Keywords: Idiosyncratic drug reactions human leukocyte antigen molecular ...
9. A method for HLA typing HLA-A with high resolution, comprising: generating HLA-A subgroups A_O using the method of claim 1, further analyzing positions 224, 268, 376, 502, 561 and 616 to resolve subgroup HLA-A_A; positions 126 and 526 to resolve subgroup HLA-A_B; positions 81,...