Methods HK2 cells were randomly divided into three groups (n=6): high glucose group (HG), high glucose hypoxia/reoxygenation group (HH/R), high glucose hypoxia / reoxygenation chloroquine preconditionning group (HH / RCQ). The high glucose and h...
Hsa-miR-494-3p also increased a hypoxia-reoxygenation induced inflammatory response in HK2 cells. Moreover, as a target gene of miR-494-3p, overexpression of HtrA3 downregulated the hypoxia-reoxygenation induced inflammatory response in HK2 cells. Overexpression of hsa-miR-494-3p-induced ...
cells(HK.2).Methods:(1)Human renal proximal tubularcelllineHK-2cell wasusedas targetcell.(2)Hypoxic cultureconditionswere producedby coveringliquidparaffinwax.(3)Experimentgrouping:Tubular cellswere dividedintosix groups:Group 4hoursof hypoxia(H),group ...
摘要 Strengthened glycolysis is crucial for the macrophage pro-inflammatory response during sepsis.Activating transcription factor 4(ATF4) plays an important role in regulating glucose and lipid metabolichomeostasisin hepatocytes andadipocytes. However, its immunometabolic role in macrophage during sepsis remain...
[Abstract] Objective To explore the role and mechanism of NGAL in the process of hypoxia/ reoxygenation H/R injury in human renal tubular epithelial cell HK-2 . Methods The H/R model of HK-2 cells was established in vitro oxygen concentration < 0. 1% . The expressions of NGAL in the ...
First, we established MCF7 breast cancer cells stably expressing YAP short hairpin RNAs (shRNA) via lentiviral infection. Then, the stable cell lines were exposed to CM from cultured human THP-1 macrophages. The ability of cell migration was measured by transwell assay. The results showed that...
PKM2 interacts with hypoxia-inducible factor 1 (HIF-1)α to enhance the expression of glycolytic genes and glycolysis in cancer cells. In addition, PKM2 binds to OCT4 to modulate Oct4-mediated tumor cell stemness. PKM2 was also found to interact with c-Rel and nuclear factor erythroid 2-...
通信作者:丁小兵 E-mail: 563109451@qq.com 482 欧阳晓春,等 ARHGAP4通过调控HK2表达促进肝癌细胞生长的研究 carcinoma cells stably expressing high levels of ARHGAP4. Western blot was used to analyze the expression of HK2, and EdU was used to analyze the proliferation of hepatocellular carcinoma ...
undernormoxiaandhypoxia were extracted and verified by RT-PCR. The elevated miRNAs under hypoxic conditions were selected and analyzed by bioinformatics analysis to find targeted genes. 3. HK2 cells were transfected with miRNA-125-5p mimics(miR-MI) and microRNA negative control(miR-NC) respectively...
byhypoxia orischemia,and itCall promote expression ofitsdownstream genes.HIF一1playsimportant rolein many physiologicalprocess,such as glyeolysis,utilization of oxygen in cells, erythrogenesis,transportation of glucose andhas significantprotective effect for kidIley cells.Tubular epithelial cellsof kidney ...