网络出版时间: 网络出版地址:◇实验方法学◇VSVG/HIV1NL43Luc假病毒筛选抗HIV 1药物的条件优化及应用王 萍1,2,陈欢2,3,罗荣华2,卿晨1,张高红2,郑永唐2(1.昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明
Interestingly, we show here that infection of CD4+ T cells with HIV-1 NL4-3, but not IIIB, inhibited activation and expression of JAK3. NL4-3-infected T cells were unable to upregulate JAK3 expression following stimulation through TCR/CD3. In addition, NL4-3, but not IIIB, inhibited ...
网络出版时间:2016-2-2610:20 网络出版地址:http://www.cnki.net/kcms/detail/34.1086.R.20160226.1020.052.html ◇实验方法学◇ VSVG/HIV1 NL43 Luc 假病毒筛选抗HIV1药物的条件优化及应用 王萍 1,2 ,陈欢 2,3 ,罗荣华 2 ,卿晨 1 ,张高红 2 ,郑永唐 2 (1.昆明医科大学药学院暨云南省天然药物药理...
的表达水平与加入的病毒量呈剂量依赖关系.比较3种实验条件下不同阳性药物抑制VSVG/HIV-1_(NL4-3)Luc的半数有效剂量浓度EC50,发现第3种条件最优.而用该假病毒对合成化合物进行筛选,发现其EC50与病毒HIV-1_(ⅢB)所得EC_(50)基本一致.结论成功建立并优化了基于VSVG/HIV-1_(NL4-3)Luc假病毒的抗HIV药物筛选...
To understand how different cell types might influence the generation of viral variants, we have examined the differences in the viral life cycle of the HIV-1 isolate, NL4-3, in the human promyelocytic cell line, HL-60, and the human T cell line, H9. NL4-3 harvested from H9 cells produ...
EcoHIV/NL4-3 is a chimeric human immunodeficiency virus type 1 (HIV-1) that can productively infect mice. This study tests the utility of EcoHIV/NL4-3 infection to reveal protective immune responses to an HIV-1 vaccine. Immunocompetent mice were first immunized with VRC 4306 which encodes su...
HIV-1 Vpu targets the host cell proteins CD4 and BST-2/Tetherin for degradation, ultimately resulting in enhanced virus spread and host immune evasion. The discovery and characterization of small molecules that antagonize Vpu would further elucidate the contribution of Vpu to pathogenesis and lay the...
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smithii archaeosomes-entrapped mzNL4-3 VLPs as a promising immunogen which specifically induces and augments T-helper 1 oriented responses against HIV antigens. 展开 关键词: VLP T helper 1 Methanobrevibacter smithii HIV-1 Cell mediated immunity Archaeosome ...