HIV protease, the enzyme that HIV needs to make new virus, is responsible for processing a couple of polyprotein gene products into mature and functional proteins. In essence, some large viral proteins must be broken down to smaller proteins with regulatory functions by the protease. It plays a...
The HIV-1Tat(transactivator of transcription) gene codes for a 14-kDa protein and as its name suggests, it is a key activator of HIV-1 transcription. It is one of the first proteins to be expressed after infection occurs. Unlike typical transcription factors that are DNA binding proteins, T...
Human immunodeficiency virus-1 (HIV-1) Tat protein is one of the most important regulatory proteins for viral gene expression in the host cell and can modulate different cellular processes. In addition, Tat is secreted by the infected cell and can be internalized by neighboring cells; therefore,...
(bound Z3 of 4 Env proteins: Consensus (ConS), Clade A (A244), B (MN), and C (1086) gp120 Envs13); (iii) part of an isolated clonal B-cell lineage,4 included to study lineage evolution and affinity maturation; and (iv) gp41-specific mAbs with strong binding to C.1086gp140 ...
The purity of all proteins was assessed by using sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) and western blotting (WB), as described in Majeed et al.16. Assessing the protein oligomeric state using size exclusion chromatography We used size exclusion chromatography (SEC) to assess the ...
公开/公告日期: 2005-03-22 申请(专利权)人: FONDAZIONE CENTRO S. RAFFAELE DEL MONTE TABOR 发明人: A Beretta 摘要: Immunological homologous epitopes of HLA and HIV virus proteins, particularly gp160, are described, to be used for diagnostics and for producing vaccines.收藏...
“The antibodies used in the crystallography study are ones that we observed to stop the dance of the HIV envelope proteins, pushing the trimer assembly into a quiescent, ground state,” Blanchard said. Both imaging techniques could help scientists describe the functions of molecules from the per...
Genetic variation occurs within LTR binding sites where host transcription factors and viral regulatory proteins bind, altering the way the LTR drives viral transcription. The resultant viral quasispecies are likely shaped by the selective pressures operative within a variety of cellular and tissue niches...
mainly located in regions III and V were obtained in this study [44]. Two later studies of the 86-residue Tat Bru [36] and the 87-residue Tat Mal [37] were performed in the absence of reducing agents and over 270 long-range NMR constraints were found in each. Both Tat proteins displ...
HIV-1 proteinase processes its virally encoded polyproteins into mature structural proteins and enzymes that are essential for viral propagation. As a consequence the proteinase is an attractive target for prospective antiviral agents for the treatment o