为了确保保护效果确实来自CCR5编辑而非编辑过程的副作用，研究人员用CCR5编辑小鼠再次进行挑战。尽管这些小鼠成功抵抗了之前的HIVJRCSF感染，但当用替代共受体CXCR4的HIV株（HIVNL43）进行挑战时，小鼠成功感染并表现出强烈的HIV病毒血症。这证明CCR5编辑小鼠的抗HIV效果是特定于CCR5表达去除的。这些结果表明，通过高频CCR5...
为了确保保护效果确实来自CCR5编辑而非编辑过程的副作用,研究人员用CCR5编辑小鼠再次进行挑战。 尽管这些小鼠成功抵抗了之前的HIVJRCSF感染,但当用替代共受体CXCR4的HIV株(HIVNL43)进行挑战时,小鼠成功感染并表现出强烈的HIV病毒血症。这证明CCR5编辑小鼠的抗HIV效果是特定于CCR5表达去除的。 这些结果表明,通过高频CCR5...
Thymic T cells expressing NL43 Nef show altered activation responses. In contrast, Nef protein of the HIV-1 HxB3 isolate does not have an overt effect on T cells when expressed in transgenic animals. The differential effects of the two HIV-1 nef alleles in transgenic mic...
网络出版时间:2016-2-2610:20 网络出版地址:http://www.cnki.net/kcms/detail/34.1086.R.20160226.1020.052.html ◇实验方法学◇ VSVG/HIV1 NL43 Luc 假病毒筛选抗HIV1药物的条件优化及应用 王萍 1,2 ,陈欢 2,3 ,罗荣华 2 ,卿晨 1 ,张高红 2 ,郑永唐 2 (1.昆明医科大学药学院暨云南省天然药物药理...
aATP1A1 expression level was measured by western blot of cell lysates of CD4+T memory cells.bCD4+T cells were pretreated with 50 nM digoxin for 2 h followed by infection with HIVNL-43(M.O.I = 0.1). The infected CD4+T cells were incubated with 50 nM digoxin for 12 days...
gb|M19921.1|HIVNL43Human immunodeficiency virus type 1, NY5/BRU (LAV-1) recombinant gb|AF324493.1|AF324493HIV-1 vector pNL4-3, complete sequence gb|EU541617.1|HIV-1 clone pIIIB from USA, complete genome gb|L31963.1|HIVTH475AHuman immunodeficiency virus type 1 (individual isolate: TH4-7-...
http://www.iias.nl/iiasn/31/IIASN31_27.pdf, Liu H, Feng T, Ha T, Cai Y, Liu X, Li J: Chinese Culture, Homosexuality Stigma, Social Support and Condom Use: A Path Analytic Model. Stigma Res Action. 2011, 1 (1): 27-35. Article CAS PubMed PubMed Central Google Scholar Zhang...
Pharr JR, Lough NL, Ezeanolue EE. Barriers to HIV Testing Among Young Men Who Have Sex With Men (MSM): Experiences from Clark County, Nevada. Glob J Health Sci. 2015;8(7):9. ArticlePubMedPubMed CentralGoogle Scholar Rodriguez-Hart C, Bradley C, German D, Musci R, Orazulike I, Ba...
为了选择具有6抗性的病毒株,我们在细胞培养中对HIV-1 NL4.3进行筛选(补充方法),得到样品后测试其他药物对具有6抗性的病毒株的活性变化选择的A128T突变株和HIV-1 NL4.3的分子克隆对雷替格拉韦或AZT的抑制敏感性没有降低。同样,雷替格拉韦和AZT对6耐药菌株都保持了充分的活性,再次证明了两种整合酶抑制剂的不同作用...