NSD2 (nuclear receptor binding SET domain protein 2) is a H3K36 methyltransferase (HKMT) required for normal hematopoietic development and essential for B cell differentiation and mature B cell function. Knockou
F, linear correlation is observed between the WT NSD2 enzyme concentration and methyltransferase activity (mean ± S.D.; n = 8). NSD2 has been implicated as a therapeutic target for a variety of cancers. Because the gene is located within the Wolf-Hirschhorn syndrome critical region of ...
This antibody is expected to cross react with isofomrs 1, 2, and 4 based upon immunogen design. 免疫原 KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone-lys N-methyltransferase NSD3. 应用 Detect Histone-lysine N-methyltransferase NSD3 using this rabbit...
In mammals, the methyltransferases responsible for H3K36 methylation include nuclear receptor binding SET domain protein 1 (NSD1)/KMT3B, NSD2/KMT3G, NSD3/KMT3F, ASH1-like histone lysine methyltransferase (ASH1L)/KMT2H, SMYD2/KMT3C, SET domain-containing protein 2 (SETD2)/KMT3A, SETD3 and...
The EZH2 (enhancer-of-zest homolog 2) subunit (also known as KMT6A) within PRC2 (polycomb repressive complex 2) complex, an evolutionarily conserved class of polycomb group proteins, is an H3K27 methyltransferase153 responsible for all three states of H3K27 methylation.154–156 The mammalian ...
A. et al. Kinetic isotope effects reveal early transition state of protein lysine methyltransferase SET8. Proc. Natl. Acad. Sci. USA 113, E8369–E8378 (2016). 13. Poulin, M. B. et al. Transition state for the NSD2-catalyzed methylation of histone H3 lysine 36. Proc. Natl. Acad. ...
39H1/2 to produce H3K9me3.100Another H3K9 methyltransferase, a heterodimer of G9a and GLP (G9a-GLP), mono- and dimethylates H3K9 in euchromatin regions to repress gene expression.101When either G9a or GLP is deleted, H3K9me1 and H3K9me2 levels are reduced in euchromatin. Interestingly,in ...
This is due to a domain containing ankyrin repeating at the noncatalytic N-terminal region in G9a, responsible for G9a’s nuclear import, and protein–protein interactions. The hydrophobic cages of the ankyrin repeat domain bind H3K9me1/2 and are not required for histone methyltransfer...
et al. NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming. Mol. Cell 44, 609–620 (2011). CAS PubMed PubMed Central Google Scholar Nimura, K. et al. A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf–Hirschhorn syndrome. Nature 460, 287–291...
PRDM PR domain-containing protein TCEP Tris(2-carboxyethyl)phosphine ITC isothermal titration calorimetry Bis-tris propane 1,3-bis[tris(hydroxymethyl)methylamino]propane HMT histone methyltransferase NSD nuclear receptor SET domain Bicine N,N-bis(2-hydroxyethyl)glycine DMSO dimethyl sulfoxide. ...