In order to compare the influence of H3K27 and H3K9 methyltransferases in controlling the HIV-1 gene silencing, researchers evaluated the effect of two inhibitory enzymes, Polycomb Repressive Complex 2 (PRC2) and euchromatic histone-lysine-N-methyltransferase 2 (EHMT2). The role of EZH2...
In mammals, the methyltransferases responsible for H3K36 methylation include nuclear receptor binding SET domain protein 1 (NSD1)/KMT3B, NSD2/KMT3G, NSD3/KMT3F, ASH1-like histone lysine methyltransferase (ASH1L)/KMT2H, SMYD2/KMT3C, SET domain-containing protein 2 (SETD2)/KMT3A, SETD3 and...
Intriguingly,in vitrohistone methyltransferase assays have demonstrated that Dot1 proteins preferentially methylate nucleosomal substrates rather than free histone H3.214,220,221In addition, it has been shown that a positively charged region within human DOT1L (amino-acid residues 390–407) is required f...
F, linear correlation is observed between the WT NSD2 enzyme concentration and methyltransferase activity (mean ± S.D.; n = 8). NSD2 has been implicated as a therapeutic target for a variety of cancers. Because the gene is located within the Wolf-Hirschhorn syndrome critical region of ...
In animals, PcG and trxG proteins form higher order protein complexes that contain SET domain proteins with histone methyltransferase activity, and are responsible for the different types of lysine methylation at the N-terminal tails of the core histone proteins. However, whether H3K4 methyltransferase...
expression of stemness genesand differentiation-specific genes. In support with this, during ES celldifferentiation, ASH2L and WDR5 levels are down-regulated whereas MLL1 and MLL3are up-regulated [47,121]. In addition, some H3K4 methyltransferase complexes may havenon-redundant cellular function ...
Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor H... The abundant and chromatin-associated protein HCF-1 is a critical player in mammalian cell proliferation as well as herpes simplex virus (HS...
Experimental & Molecular Medicine Histone lysine methylations K Hyun et al 131 Nishida M, Kato M, Kato Y, Sasai N, Ueda J, Tachibana M et al. Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a. Genes Cells 2007; 12: 877–888. 132 Gupta-Agarwal S,...
Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase Plant Mol. Biol., 27 (1995), pp. 249-261 View in ScopusGoogle Scholar Kohler and Milstein 1975 G. Kohler, C. Milstein Continuous cultures of fused cells secreting antibody ...
However, trimethylation of the H3K27 site (H3K27me3) by Enhancer of zeste homolog 2 (EZH2), a histone methyltransferase, which is one of the components of the Polycomb repressor complex (PRC), leads to gene silencing [24]. Thus, inhibition of CBP HAT has the potential to modulate the ...