Histidine tagging and purification of tobacco etch potyvirus helper component protein - Blanc, Dolja, et al. - 1999 () Citation Context ...om which infectious transcripts can be derived (Dolja et al., 1992), an alternative purification procedure involving fusion of a histidine-tag to the 59...
The introduction of site-specific labeling methods usingenzymeslikesortasehas also allowed for easy manipulation of recombinant antibodies for site specific tagging. This was demonstrated when recombinant antibodies were cloned with the peptide acceptor motif (LPXTG) of sortase to allow covalent attachment ...
Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA chromatography.This is a preview of subscription content, log in via an institution to check access. Similar content being viewed by others...
Expression and Characterization of Recombinant Caveolin PURIFICATION BY POLYHISTIDINE TAGGING AND CHOLESTEROL-DEPENDENT INCORPORATION INTO DEFINED LIPID ME... Caveolin, a 22-24-kDa integral membrane protein, is a principal component of caveolar membranes . Caveolin has been proposed to function as a scaf...
6-Histidine (Epitope Tagging)推荐供应商 我要询价 上海优宁维生物科技股份有限公司 联系电话:021-38939000-9068 电子邮件:dongh@univ-bio.com CB指数:58 网址:https://www.univ-bio.com/ 相关信息:产品目录(6647) 用户评价 英文名称:6-Histidine (Epitope Tagging) ...
Head or ear blight, mainly caused by Fusarium species, can devastate almost all staple cereal crops (particularly wheat), resulting in great economic loss and imposing health threats on both human beings and livestock1–3. However, achievement in breedin
Site-specific Fluorescent Labeling of Poly-histidine Sequences Using a Metal-chelating CysteineSite-specific Fluorescent Labeling of Poly-histidine Sequences Using a Metal-chelating CysteinecysteinedibromobimanefluorescenceHis‐tagmetal chelatingprotein taggingCoupling genetically encoded target sequences with speci...
The present invention describes how his-tagging greatly simplifies and expedites purifying Shiga toxins, and describes an improved method for such purification. One aspect of the invention is obtaining and using Shiga toxoids that are immunoreactive but not toxic. Another aspect of the invention is ...
C-terminal glycine-histidine tagging of the outer membrane protein Igaβ of Neisseria gonorrhoeae . FEMS Microbiol. Lett. 127 : 249-254.Strauss et al., " C-terminal glycine-histidine tagging of the outer membrane protein Iga beta of Neisseria gonorrhoeae " FEMS Microbiol. Lett. 127(3):249-...
Young-Hee ShinHee Sook KimDong Hee NaJournal of Pharmaceutical InvestigationH. Song, E. Park, Y.-H. Shin, H. Kim, and D. Na, "Effect of polyhistidine-tagging site on the stability of recombinant alginate lyase from Streptomyces sp. ALG-5," Journal of Phar- maceutical Investigation, ...