Pirone.Histidine-tagging and purification of tobacco etch potyvirus helper component protein. Journal of Virology . 1999Sthane Blanc Valerian V. Dolja Cesar Llave and Thomas P. Pirone.Histidine-tagging and purification of tobacco etch potyvirus helper component protein. Journal of Virology . 1999...
Besides IMAC, liquid-liquid extraction with affinity ionic liquids has been also used for the same purpose, resulting in a suitable method for large-scale purification (Ren et al., 2015). His-tagging has served for the purification of structures more complex than single polypeptides, such as ...
limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA ...
Hexahistidine tagging of proteins for purification by means of immobilized metal ion chromatography has been used for a number of proteins expressed in bacteria (12, 13), Sf9 cells (14, 15), and mammalian cell lines (16). The hexahistidine tag allows for a fast and efficient one-step puri...
tagginghistidineaffinity purificationrecombinant proteinnickelExpression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant...
C-terminal glycine-histidine tagging of the outer membrane protein Igaβ of Neisseria gonorrhoeae . FEMS Microbiol. Lett. 127 : 249-254.Strauss et al., " C-terminal glycine-histidine tagging of the outer membrane protein Iga beta of Neisseria gonorrhoeae " FEMS Microbiol. Lett. 127(3):249-...
Such a HIS-tag typically consists of six histidine residues placed in either the N- or C-terminus of the protein. Histidine tagging enables efficient protein purification on an affinity column by chelation between the histidine residues and fixed transition metals such as Cu(II) and Ni(II) [4...
The introduction of site-specific labeling methods usingenzymeslikesortasehas also allowed for easy manipulation of recombinant antibodies for site specific tagging. This was demonstrated when recombinant antibodies were cloned with the peptide acceptor motif (LPXTG) of sortase to allow covalent attachment ...
Tagging of protein C-termini with poly-arginine or poly-lysine tags raises the isoelectric point but was reported to facilitate purification via conventional and cost-effective cation-exchange chromatography [10], [11], [12], [13], [14], [15], [16]. A comparative study of the purification...
In yet another embodiment, the 3-frame His-tag DNA sequence of this invention is used in activation tagging vectors. An activation tagging vector containing a 3-frame His-tag coding sequence can be introduced into an organism and allowed to randomly insert into the genome. The organism is then...