蛋白和金属离子之间的结合强度受几种因素影响,包括长度、位置、亲和标记在蛋白的暴露程度、所用离子的类型、以及缓冲液的pH,因此一些蛋白用其他离子可能更容易地进行纯化而不用Ni2+。可以利用Hitrap IMACHP来筛选不同的金属离子,具体应用实例请参考《Recombinant Protein Purification Handbook》。 若没有洗脱下来,请依次...
蛋白和金属离子之间的结合强度受几种因素影响,包括长度、位置、亲和标记在蛋白的暴露程度、所用离子的类型、以及缓冲液的pH,因此一些蛋白用其他离子可能更容易地进行纯化而不用Ni2+。可以利用Hitrap IMACHP来筛选不同的金属离子,具体应用实例请参考《Recombinant ...
If you wish to purify your protein of interest from higher amounts of starting material, you may need to optimize the lysis protocol and purification conditions (amount of resin used for binding). The optimization depends on the expected yield of your protein and amount of resin to use for ...
Brochure: Tools and reagents for recombinant protein purification KingFisher Flex Bindt Software protocol for His-tagged fusion protein purification or pull-down Product Information User Guide: HisPur Ni-NTA Magnetic Beads 引用和文献(3) 搜索
This protocol provides simple, rapid purification of his-tagged proteins from up to 800 µl of clarified lysate from mammalian or bacterial cell samples using theCapturem His-Tagged Purification Miniprep Kit. The columns are suitable for use under native or denaturing conditions, ...
Among the main drawbacks with these systems are that the affinity tag may affect protein structure and function, and that it 1s not possible to purify insoluble proteins.doi:10.1385/0-89603-485-2:293Richard E. KneuselJoanne CroweMelanie Wulbeck...
cOmpleteHis-TagPurification-罗氏His标签纯化
Protocol: denaturing purification with TALON resin, imidazole elution Protocol: native purification with TALON resin, imidazole elution Protocol: native purification with TALON resin, pH elution New products Get high yields of highly concentrated his-tagged proteins, in only 5 minutes, with theC...
Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the HisPur Ni-NTA Magnetic...
After purification, I can detect my protein by western blotting using the His-tag antibody but can also see additional bands running lower. What might they represent? They may represent cleavage products of the recombinant protein. Unspecific protein cleavage can occur during protein production (e....