The availability of purified and active protein is the starting point for the majority of in vitro biomedical, biochemical, and drug discovery experiments. The use of polyhistidine affinity tags has resulted in great increases of the efficiency of the protein purification process, but can negatively...
20; detergent, which can interfere with accurate mass spectrometry. find additional tips, troubleshooting help, and resources within our protein assays and analysis support center . this principle is based on the reversible interaction between various amino acid side chains and immobilized metal ions. ...