From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein PurificationProtein purification often requires multiple iterations of individual column purifications, sample fractionation andvisualization, and fraction pooling for the next column. Significant time is spent developing the ...
Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification. EDTA-resistant resin for IMAC chromatography Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a c...
Polyhistidine tag, comprising of six histidine residues, is commonly used for affinity purification, and longer, as well as shorter tags, have also been used successfully. The polyhistidine tag may be placed at the N- or the C-terminal of the protein. The tag may also be placed internally...
Secondly, the separation and purification of recombinant fusion protein rely on all kinds of protein tags [3], [4], [5], which can chelate with metal ions on solid resin. Among affinity tags, the most common affinity tag is polyhistidine, which can bind to immobilized Ni2+ or Co2+ ...
protein (Maseko et al., 2019;Nguyen et al., 2015). To overcome this challenge, the His-tag can be combined with solubility enhancing tags (likeMBP,GST, and Trx) to increase the solubility of HIV-1 PR and at the same increase the purification efficiency (Azarnezhad et al., 2016;Maseko...
Thromboxane A2(TxA2) receptors belong to the class of G-protein-coupled receptors. Knowledge of the relationship of structure to function for TxA2 receptors is limited because of their low levels of expression, lengthy purification procedures and poor recoveries. A C-terminal hexahistidine-tag (C-...
For fusing the antigenic HSV-tag to pSRII, pSOPII was amplified as described above and ligated into the NcoI/EcoRI restricted vector pET27bmod. DNA sequencing was carried out using the Dye Didesoxy kit from Applied Biosystems (CA). 2.3. Protein expression and purification Transformed E. coli...
Owing to the characteristic molecular recognition ability between nickel(II) ions and the polyhistidine affinity tag, the nanoparticles could be successfully employed to selectively bind and separate His-tagged cyan fluorescent protein (CFP) from an E. coli cell lysate in a recyclable process. More...
An ideal affinity tag should enable effective but not too strong a binding, and allow elution of the desired protein under mild, nondestructive conditions. In the common chelating ligands, because of the 3-dentation, the binding force of iminodiacetic acid (IDA) with the metal ion is weak. ...
Owing to the characteristic molecular recognition ability between nickel(II) ions and the polyhistidine affinity tag, the nanoparticles could be successfully employed to selectively bind and separate His-tagged cyan fluorescent protein (CFP) from an E. coli cell lysate in a recyclable process. More...