aMetal chelators as affinity ligands for His-tagged proteins 金属螯合剂当亲合力ligands为他被标记的蛋白质 [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51La ...
Betz N (2004) Purifying His-tagged proteins from insect and mammalian cells. Cell Notes 9:6–9Betz, N "Efficient purification of his-tagged proteins from insect and mammalian cells". Promega Notes 87, 29-32, 2004.Betz N: Efficient purification of His-tagged proteins from insect and ...
His-tagged fusion protein purification can be used as the only purification step for applications that do not require proteins with extremely high purity. When the highest purity is needed, this technique can be used as the first (capture) step in a multistep purification procedure. It is also...
TALON Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including: Microscale purification of his-tagged proteins Studying protein structure and function Preparing proteins for X-ray crystallography Performing ...
Covalently derivatized NTA microarrays on porous silicon for multi-mode detection of His-tagged proteins[J]. PEI Jia 1 ,TANG YanChun 3 ,XU Ning 1 ,LU Wei 3 ,XIAO ShouJun 1,2* &LIU JianNing 3* 1 State Key Laboratory of Coordination Chemistry;Nanjing National Laboratory for Microstructures;...
Histidine-tagged protein purification can be used to purify proteins expressed as inclusion bodies. How can I remove a histidine-tag? The histidine-tag is small, so it usually does not have to be removed. Remove the tag if: it interferes with the function of the target protein ...
BenchMark His-tagged Protein Standard is designed as a positive control and for molecular weight sizing in his-tagged fusion protein detection. Each of the ten standard proteins (10-160 kDa) contains a 6X His-tag, enabling the detection of standard proteins with the InVision™ His-tag In-ge...
Conventional methods for purifying recombinant his-tagged proteins involve immobilized metal affinity chromatography (IMAC) columns and a lengthy procedure that can require desalting and buffer exchange before loading the column, as well as numerous column washes. By contrast, the...
However, switching the metal to Cu(II) instead of Ni(II) brings an excellent opportunity for releasing the His-tagged proteins by the reduction of Cu(II). The affinity of Cu(I) towards complexation with NTA and His-tag is substantially lower than Cu(II) which facilitates the release of ...
This protocol provides simple, rapid purification of his-tagged proteins from up to 800 µl of clarified lysate from mammalian or bacterial cell samples using theCapturem His-Tagged Purification Miniprep Kit. The columns are suitable for use under native or denat...