Purification of His-tagged proteins. SPRIESTERSBACH A,KUBICEK J,SCHAFER F,et al. Methods in Enzymology . 2015Spriestersbach A, Kubicek J, Schafer F, Block H, Maertens B. Purification of His-Tagged Proteins. In: Lorsch JR, editor. Laboratory Methods in Enzymology: Protein, Pt D. Methods...
His-taggedproteinbytheresin. 2.5Washthecolumnwith10timesasmuchvolumeofthewash bufferasthecolumnvolume. Note:Washingwithwashbuffercontainingahigher concentrationofimidazolecanincreasethepurityofa recombinantHis-taggedprotein,althoughitdecreases theyield.
magnetic separator. The beads, which are precharged with Co2+, have a higher specificity for his-tagged proteins than nickel-based resins. Co2+is bound to the beads using TALON's unique tetradentate metal chelator, which binds cobalt at four sites, virtually eliminati...
Reversible immobilization of rhamnulose-1-phosphate aldolase for biocatalysis: Enzyme loading optimization and aldol addition kinetic modeling histidine residues of proteins with the metal chelated in affinity supports, has been extensively exploited in purification of His-tagged proteins in one-... I Ar...
Capturem his-tagged purification protocols Visual protocol: Capturem his-tagged miniprep kit See how you can perform simple, rapid purifications of his-tagged proteins. Visual protocol: Capturem his-tagged maxiprep kit See how you can purify his-tagged proteins from up to 25 ml of clarified...
PhyTip® Columns for Automated Purification of Tagged Proteins Recombinant proteins can be expressed carrying an affinity tag that can be used for affinity purification. The choice of affinity tag can influence the protein solubility, stability and biological activity. Our unique PhyTip columns enable...
Hengen P. Purification of His-Tag fusion proteins from Escherichia coli . Trends Biochem Sci. 1995; 20 :285–286. doi: 10.1016/S0968-0004(00)89045-3. [ Cross Ref ]Hengen P (1995) Purification of His-Tag fusion proteins from Escherichia coli. Trends Biochem Sci 20:285-286...
His Tag Protein Purification Protocol Histidine-tagged proteins can be purified from a wide range of expression systems under native or denaturing conditions. The main drawback is that the technique often requires optimization to minimize nonspecific binding of host cell proteins. ...
ligands for the purification of his-tagged proteins (Ni-NTA, Ni-IDA, and Cobalt), GST-tagged proteins (Glutathione), c-Myc-tagged proteins (anti-c-Myc), HA-tagged proteins (anti-HA), and our recently launched supports to purify DYKDDDDK (FLAG)-tagged protein (Anti-DYKDDDDK...