His Tag Protein Purification Protocol Histidine-tagged proteins can be purified from a wide range of expression systems under native or denaturing conditions. The main drawback is that the technique often requires optimization to minimize nonspecific binding of host cell proteins. ...
To this end, Pro-Chem is heavily committed to method development andextending the knowledge of applications of these spin columns. All protocols are rigorouslyevaluated against a panel of protein samples and test conditions to ensure that the kits comply with all published specifications and exceed ...
20061 GeneralRemarks Thisprotocolisbasedonthepurificationprocedureusedwiththe automaticproteinsynthesizerProtemist®DT,butitcanbeusedfor manualpurificationaswell. Abbreviations:GFP,GreenFluorescenceProtein;GUS,-D-glucuronidase; Akt1,v-aktmurinethymomaviraloncogenehomolog1;ERK2,Extracellular signalRegulatedKinase2;...
His标签蛋白纯化试剂盒(包涵体蛋白)价格:价格电议产品详情:His标签蛋白纯化试剂盒(包涵体蛋白)中文名称:His标签蛋白纯化试剂盒(包涵体蛋白)英文名称:His-Tagged Protein Purification Ki... 货号:LM-0266 品牌:上海联迈生物工程有限公司 产地:中国/上海 保质期:一年 ...
Protocol: native purification with TALON resin, pH elution VIDEO PROTOCOL Next-generation his-tagged protein purification in a maxiprep format Get high yields of highly concentrated his-tagged proteins, in only 15 minutes, with theCapturem His-Tagged Purification Maxiprep Kit. ...
His-Tagged Protein Purification Kit (Inclusion Body Protein) 产品货号:CW0893S 产品规格:5ml His标签蛋白纯化试剂盒(包涵体蛋白) 该产品为镍柱纯化系统,对6×His-tag蛋白具有显著特异吸附能力,能够高效一步纯化带有6个组氨酸亲和标签的蛋白。该系统具有4个Ni2+螯合位点,较只有3个螯合位点的Ni-IDA结合Ni2+更为牢...
英文名称:His-Tagged Protein Purification Kit(Soluble Protein) 产品货号:WE0267 产品规格:5ml ??本试剂盒包含Ni-Agarose填料、亲和柱空柱以及可溶性His融合蛋白纯化所需的全部试剂(细菌裂解液、蛋白酶抑制剂混合物、结合缓冲液和洗脱缓冲液组分),使用方便。该镍柱纯化系统对6×His-tag蛋白具有显著特异吸附能力,能够...
This protocol provides simple, rapid purification of his-tagged proteins from up to 800 µl of clarified lysate from mammalian or bacterial cell samples using theCapturem His-Tagged Purification Miniprep Kit. The columns are suitable for use under native or denat...
This protocol provides simple, rapid purification of his-tagged proteins from up to 800 µl of clarified lysate from mammalian or bacterial cell samples using theCapturem His-Tagged Purification Miniprep Kit. The columns are suitable for use under native or denaturing conditions, a...
Tagged Protein Purification Kit (Soluble Protein) His标签蛋白纯化试剂盒(可溶性蛋白) 5ml 2-8℃ 否蛋白提取相关试剂盒 His-Tagged Protein Purification Kit (Soluble Protein) His标签蛋白纯化试剂盒(包涵体蛋白) 5ml 2-8℃ 否蛋白提取相关试剂盒 昆虫蛋白提取试剂盒 50T -20℃ 否蛋白提取相关试剂盒 昆虫蛋白...