本研究创新性地发现PRPF31基因缺陷型视网膜色素上皮细胞(hiPSC-RPE)分泌的细胞外囊泡(EVs)具有独特的RNA特征谱,鉴定出18种miRNA和865种poly(A)RNA可作为视网膜退行性病变的潜在循环生物标志物。作者通过对比基因编辑PRPF31+/+与PRPF31+/−模型,首次系统揭示了EVs RNA cargo与RPE功能状态的分子关联,为遗传性视网...
Conversely, although the RPE sheet ensures targeted delivery with correct cell polarity, it requires invasive surgery, and graft preparation is time-consuming. We previously reported hiPSC-RPE strips as a form of quick cell aggregate that allows for reliable cell delivery to the target area with ...
目的: VKH(Vogt-小柳原田综合征)是我国常见且致盲率较高的葡萄膜炎.尽管已广泛研究了VKH的发病机理,但仍不清楚VKH的确切病因.目前的研究是分离VKH患者外周血单核细胞,建立诱导性多能干细胞,再将其诱导分化为iPSC-RPE,为后期探讨VKH的发病机制及药物筛选提供细胞模型. 方法: 从具有VKH诊断的患者和正常对照者的外周...
hiPSC-derived RPCs and RPE cells expressing (GFP) were transplanted into the subretinal space of rats.doi:10.1016/J.OMTM.2021.02.006Anna SalasAnna DuarriLaura FontrodonaDiana Mora RamírezAnna BadiaHelena Isla-MagranéBarbara Ferreira-de-Souza
Building on our prior work with human induced pluripotent stem cell derived (hiPSC-)RPE strips, a rapid cell aggregate form, this study aims to evaluate their effectiveness in a microsecond pulse laser-induced local RPE ablation model in cynomolgus monkey eyes. Methods : A local RPE ablation ...
The location of hiPSC-RPE cells can be distinguished by employing gold nanochains. Over a period of 8 months, labeled cells can be tracked within the subretinal space. This imaging platform holds promise as a valuable tool for investigating cell-based therapeutic interventions in various eye ...
本发明涉及一种结合生物基质胶生成hiPSCRPE细胞膜片的方法,包括,步骤S1,将预处理后的猪眼葡萄膜制备脱细胞基质胶;步骤S2,维持培养hiPSCs;步骤S3,培养hiPSCRPE悬浮球;步骤S4,hiPSCRPE细胞扩增培养;步骤S5,生成hiPSCRPE细胞膜片;其中,所述步骤S5中,将浓度为1%的UveaGel包被培养皿,于37℃放置30min左右,待UveaGel凝固,...
Finally, although serum exposure was not necessary for drusen formation, COL4 accumulation in ECM, and complement pathway gene alteration, it impacted the composition of drusen-like deposits in patient hiPSC-RPE cultures. Together, the drusen model(s) of MDs described...
Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSoxCELL sheets (Biology)TISSUE engineeringBIOPRINTINGINDUCED pluripotent stem cellsRHODOPSINSMALL moleculesBackground: Retinal pigment epithelium (RPE) cell therapy is a promising way to treat many retinal ...
This study aims to enrich and expand hiPSC-RPE acquired with this platform and explore characteristics of serially passaged RPE cells.Liu, ShengxuXie, BingbingSong, XiaojingZheng, DandanHe, LiwenLi, GuilanGao, GuanjiePeng, FuhuaYu, MinzhongGe, JianZhong, Xiufeng...