Moreover, SP1 inhibition (MIT) or SK1 inhibition (SKI-II) completely abolished the renal protective effect of hiPSC-MSCs-EVs in rat I/R injury mode. However, pre-treatment of necroptosis inhibitor Nec-1 showed no difference with the administration of hiPSC-MSCs-EVs only. We then generated ...
We compare the therapeutic efficacy and cell survival of intramuscular transplantation of hiPSC-MSCs with or without a single or repeated intravenous administration of hiPSC-MSCs. Repeated intravenous administration of hiPSC-MSCs can increase splenic regulatory T cells (Tregs) activation, decrease splenic ...
随后利用细胞追踪、活体成像、脾脏透明化等技术发现hiPSC-MSCs在脾脏聚集,与脾神经末梢定位接近。研究团队进一步构建切除脾神经的SHR模型、谷氨酸合成受抑制的hiPSC-MSCs细胞系,证实hiPSC-MSCs可通过旁分泌谷氨酸激活脾神经,促进其释放去甲肾上腺素。后续利用AAV构建ChAT+细胞缺陷的SHR模型,证实脾神经通过激活脾脏ChAT+细胞...
上海交通大学附属第六人民医院科研团队研究发现hiPSC-MSCs外泌体在体外以剂量依赖性方式刺激人真皮成纤维细胞和 HUVEC 的增殖和迁移。同样,随着 hiPSC-MSC-Exos 浓度的增加,I、III 型胶原蛋白和弹性蛋白的分泌以及成纤维细胞的 mRNA 表达和 HUVEC 的管形成也增加。 相关研究发表在J Transl Med. ,文章标题为Exosomes...