CiPA hiPSC-CMs检测 CiPA (the Comprehensive In Vitro Proarrhythmia Assay) 是US FDA, CSRC,HESI和SPS等机构提出的有关临床前药物心脏安全评估的新建议,其目的是修改原来的临床前心脏安全评估S7B规则并废除药物临床心脏安全评估的E14规则。除离子通道检测外,还增加了hiPSC-CMs检测,通过MEA与VSD对干细胞诱导心肌细胞...
hiPSC-CMs潜在应用 局限性 体外产生的hiPSC-CMs可能无法完全概括心脏的复合物体内环境,并且可能无法准确反映心脏中发现的心肌细胞亚型的多样性。此外,hiPSC-CMs可能不适用于所有类型的心血管研究,例如需要完整心脏组织或整个动物模型的研究。这时候心脏工程组织更具优势。此外,hiPSC-CMs的幼稚性问题一直是存在的研究焦点。
(hiPSC-CMs)移植到心肌梗死部位,结果表明hiPSC-CMs移植存活率较低,治疗心肌梗死的有效性因此受到了限制.如何更好地提高移植hiPSC-CMs存活率和移植率成为本研究的重点.目的:探究丹参乙酸镁是否能抑制心肌梗死后梗死部位炎症反应和抑制梗死部位移植的hiPSC-CMs的凋亡,以及是否能提高hiPSC-CMs存活率和改善心功能.此外,探究...
氯化钻诱导hiPSC-CMs体外缺氧模型的建立 朱梦怡-柯敏霞I,王皓齐念民益3,吴月红 (1.浙江理工大学生命科学与医药学院，杭州310018；2.杭州标模生物科技有限公司，杭州310018；3.上海丽坤生物科技股份有限公司，上海201499)摘要：为进一步了解缺氧性心血管疾病的发病机制.通过使用CHIR99021和IWP2抑制剂的组合诱导和单层诱导...
(hiPSC-CMs)移植到心肌梗死部位,结果表明hiPSC-CMs移植存活率较低,治疗心肌梗死的有效性因此受到了限制.如何更好地提高移植hiPSC-CMs存活率和移植率成为本研究的重点.目的:探究丹参乙酸镁是否能抑制心肌梗死后梗死部位炎症反应和抑制梗死部位移植的hiPSC-CMs的凋亡,以及是否能提高hiPSC-CMs存活率和改善心功能.此外,探究...
Methods hiPSC-CMs were cultured on; 1) a highly aligned polylactide-co-glycolide (PLGA) nanofiber scaffold (~50 microns thick) and 2) on a standard flat culture plate. Scanning electron microscopy (SEM) was used to determine alignment of PLGA nanofibers and orientation of the cells on the ...
Metabolic flux analysis in hiPSC-CMs reveals insights into cardiac dysfunction in propionic acidemia doi:10.1007/s00018-025-05661-5Propionic acidemiaHuman induced pluripotent stem cell-derived cardiomyocytesMetabolic fluxGlucose metabolismFatty acid metabolismCardiac diseasesPropionic acidemia is an inborn error...
Disrupting PAS activity increases IKr and shortens APD in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). We thus hypothesized that the hERG1 PAS domain could represent a therapeutic target to reduce arrhythmogenic potential in a long QT syndrome (LQTS) background. To test ...
Incubation with dapa or empa (24-h,1 M) significantly increased INa and IK1 densities recorded in human-induced pluripotent stem cell-cardiomyocytes (hiPSC-CMs) using patch-clamp techniques. Dapa and empa, respectively, shifted to more hyperpolarized potentials the INa activation and inactivation ...
Deciphering Role of Wnt Signalling in Cardiac Mesoderm and Cardiomyocyte Differentiation from Human iPSCs: Four-dimensional control of Wnt pathway for hiPSC-CMs differentiation Meng Zhao, Yawen Tang, Yang Zhou & Jianyi Zhang Scientific Reports volume 9, Article number: 19389 (2019) Cite this articl...