data <- read.table(files[i], header = TRUE, skip = 1) lastColumn <- data[, ncol(data)] mergedData <- cbind(mergedData, lastColumn) } #mergedata这一步是将之前提取的column1和lastColumn合并为一个新的数据框,赋值给mergedData变量。.;files <- c(file2, file3, file4):创建一个名为fil...
Broad Institute视频笔记 Introduction to High-throughput Sequencing Data 无意间发现了油管上有一个系列视频,是Broad Institute在去年发布的课程,这个课程主要是对GATK第4版进行的一个系统的介绍。所以我也是打算把这一个系列先学习一下,这样就可以对GATK有一个系统的理解了: 这个系列视频一共有17讲,当然我也不确定...
TERIUS: accurate prediction of lncRNA via high-throughput sequencing data representing RNA-binding protein association 来自 NCBI 喜欢 0 阅读量: 2035 作者:Choi, Seo-Won,Nam, Jin-Wu 摘要: LncRNAs are long regulatory non-coding RNAs, some of which are arguably predicted to have coding potential. ...
Accessing whole-genome data by next-generation sequencing methods is therefore crucial for addressing CMV molecular variation and the factors underlying its observed status, as it can provide sequence data for CMV and satellite RNAs present in samples as well as coinfecting viruses if the proper ...
As high-throughput sequencing applications continue to evolve, the rapid growth in quantity and variety of sequence-based data calls for the development of new software libraries and tools for data analysis and visualization. Often, effective use of thes
FastQC will highlight any areas where this library looks unusual and where you should take a closer look. The program is not tied to any specific type of sequencing technique and can be used to look at libraries coming from a large number of different experiment types (Genomic Sequencing, ChI...
MOTIVATION: The advent of high-throughput sequencing (HTS) technologies has made it affordable to sequence many individuals' genomes. Simultaneously the computational analysis of the large volumes of data generated by the new sequencing machines remains a challenge. While a plethora of tools are availa...
great application in high-throughput sequencing and microarray technology. Firstly,this technology uses microarray to synthesise oligonucleotide probeswhich could combined with certain parts of the genome, so that it assemble es specific segment and then use the second generation sequencing to sequencing ...
High Throughput Sequencing(HTS) technologies are recent and continuously developing techniques that enable the sequencing of multiple DNA molecules in parallel, creating large data sets in a shorter time than ever possible, moreover the costs of HTS are decreasing. These advantages have made pos...
In this study, the transcriptome was sequenced with the Illumina Hiseq sequencing platform, and an Illumina paired-end (PE) library (300 bp) was constructed for 2 × 150 bp sequencing. The sequencing data obtained were quality-tested and analyzed with bioinformatics. Library data ...