COTTONQUALITY controlKARYOTYPESCHROMATINHigh-throughput chromosome conformation capture (Hi-C) technology has been applied to explore the chromatin interactions and shed light on the biological functions of three-dimensional genomic features. However, it remains challenging to guarantee ...
Hi-C是橙汁?难道不是High-throughput/resolution chromosome conformation capture吗?我不管,反正我只看到了我家Hi-C又在促销了。对,在生物领域奋斗的我们就是不一样~我们不一样~我们不一样~ O网页链接 @界面新闻 【怀旧上瘾!四川辣酱之后美国人民又要求麦当劳复活Hi-C橙汁】麦当劳美国在1998年推出的一款用来蘸麦香...
Fig. 2 Mechanisms of enhancer action. Enhancer action on target promoters can occur via looping that enables physical contact in either a stable or dynamic manner in agreement with the evidence of proximity from chromatin conformation experiments and DNA FISH [39]. Alternative mechanism of action in...
et al. Chromosome Conformation Capture Carbon Copy (5C): a massively parallel solution for mapping interactions between genomic elements. Genome Res. 16, 1299–1309 (2006). Article CAS Google Scholar Kwasnieski, J.C., Fiore, C., Chaudhari, H.G. & Cohen, B.A. High-throughput functional...
The second major improvement shared between the techniques is the use of optimized nucleosome disruption methods which we previously described for s3-WGS (for single-cell Whole Genome Sequencing) and s3-GCC (single-cell Genome sequencing plus Chromosome Conformation)12. These improvements include a ...
An experimental structure of the KCNE1/KCNQ1/calmodulin complex has not been determined, so we examined multiple AI-based models (Fig. S11). Aligning predicted structures of KCNE1 to a reported cryo-EM structure of the KCNQ1-KCNE3 complex indicated that the conformation obtained from AlphaFold-...
Chromatin conformation capture technologies, such as Hi-C, are typically time- and resource-consuming1, limiting their contribution to understanding how chromatin organization determines cell-type-specific gene expression. Owing to its ability to model complex interactions, deep learning has emerged as a...
HP1 binds to di- or tri-methylated lysine 9 of histone H3 (H3K9me2/3) and can package nucleosomal DNA into a conformation that is able to repress transcription [3–5]. Several observations indicate that the regulatory activity of HP1 is context-dependent. In Drosophila, the archetype ...
Mutations that alter this motif, which is termed the X-region, have been shown to alter the conformation of the histidine kinase EnvZ and significantly reduce its phosphatase activity[29]. EnvZ is a membrane receptor kinase-phosphatase, which modulates porin expression in E. coli in response ...
Moreover, the protocol could also be integrated into other assays, such as DNA methylation analysis or chromatin conformation studies. The intra-nuclei chromatin barcoding strategy paves the way for combinatorial barcoding strategies, similarly to recent developments20,21,22, used for single cell ChIP...