Chromosome conformation capture (3C) technology is a pioneering methodology that allowsin vivogenomic organization to be explored at a scale encompassing a few tens to a few hundred kilobase-pairs. Understanding
Chromosome conformation capture (3C) assays are used to map chromatin interactions genome-wide. Chromatin interaction maps provide insights into the spatial organization of chromosomes and the mechanisms by which they fold. Hi-C and Micro-C are widely us
核心的技术就是3C(Chromosome Conformation Capture)实验技术。 染色质构象首先被甲醛交联固定;随后,基因组被限制性内切酶消化;存在相互作用的染色质由于空间接近,在重新连接酶消化后的基因组时,存在相互作用的染色质将被连接到一起;解交联纯化DNA后,针对感兴趣的两个特定基因组区域分别设计引物,并对重连接产物进行PCR扩...
regulationandcanbeidentifiedwiththeChromosomeConformationCapture(3C)methodology.3Cconverts physicalchromatininteractionsintospecificligationproducts,whicharequantifiedindividuallybyPCR.Herewe presentahigh-throughput3Capproach,3C-CarbonCopy(5C),thatemploysmicroarraysorquantitativeDNA sequencingusing454-technologyasdetectionmetho...
The development and widespread implementation of chromosome conformation capture (3C) technology has allowed unprecedented new insight into how chromosomes are folded in three-dimensional (3D) space. 3C and its derivatives have contributed tremendously to the now widely accepted view that genome topology ...
Recent analysis using chromatin conformation capture technology shows that members of the conserved Microrchidia ATPase family, AtMORC1 and AtMORC6 in A. thaliana, are involved in heterochromatin condensation (Moissiard et al., 2012). This suggests that plant chromosome condensation is regulated by ...
The abbreviations used are: MAR, matrix attachment region; 3C, chromosome conformation capture; qPCR, quantitative real time PCR; DMR, differentially methylated region; IG, intergenic germ line-derived; HRS, high salt recovered sequence; NC, negative control; En, embryonic day n; Dn, postnatal da...
Chromosome conformation capture-based methods Chromosomes Genetic mapping Mouse Quantitative trait Quantitative trait loci References Amar, L.C., Arnaud, D., Cambrou, J., Guenet J.L., and Avner, P.R.: Mapping of the mouse X chromosome using random genomic probes and an interspecific ...
KEGG: Kyoto Encyclopedia of Genes and Genomes DNA: Deoxyribo nucleic acid Hi-C: High-throughput chromosome conformation capture PCR: Polymerase chain reaction SNPs: Single nucleotide polymorphisms INDELs: Insertion and deletion Iso-Seq: Isoform-sequencing MYA: Million years ago ...
Huang PY, Han Y, Handoko L, Velkov S, Wong E, Cheung E, Ruan X, Wei C, Fullwood MJ, Ruan Y. Sonication-based Circular Chromosome Conformation Capture with next-generation sequencing analysis for the detection of chromatin interactions. Protocol Exchange: Protocol. 2010....