Genome-wide chromatin conformation capture assays provide formidable insights into the spatial organization of genomes. However, due to the complexity of the data structure, their integration in multi-omics workflows remains challenging. We present data
Genome-wide three-dimensional chromatin conformation capture sequencing To generate physical scaffolding information for the BAC sequence based genome assembly, as proposed in ref.21, Hi-C and tethered conformation capture (TCC) sequencing data were generated from 7-day-old leaf tissue of greenhouse-gr...
Chromosome conformation capture (3C) technology is a pioneering methodology that allows in vivo genomic organization to be explored at a scale encompassing a few tens to a few hundred kilobase-pairs. Understanding the folding of the genome at this scale is particularly important in mammals where di...
核心的技术就是3C(Chromosome Conformation Capture)实验技术。 染色质构象首先被甲醛交联固定;随后,基因组被限制性内切酶消化;存在相互作用的染色质由于空间接近,在重新连接酶消化后的基因组时,存在相互作用的染色质将被连接到一起;解交联纯化DNA后,针对感兴趣的两个特定基因组区域分别设计引物,并对重连接产物进行PCR扩...
3C: Chromosome Conformation CaptureCross-linkDigestionIntramolecular ligation3C product3C templatePCR standard templateAssociation frequencyIt is becoming increasingly apparent that is not randomly folded into the , but instead is highly organized into specific conformations within the . One consequence of ...
High-resolution chromosome conformation capture analysis IFN: Interferon KS: Klinefelter syndrome mAb: Monoclonal antibody obs/exp : Observed-to-expected ratio PBMCs: Peripheral blood mononuclear cells pDCs: Plasmacytoid dendritic cells RNA FISH: RNA fluorescence in situ hybridization TAD: Topo...
(ONT) sequencing, and high-throughput chromosomal conformation capture (Hi-C) analysis (Fig.1). The genome size of CarS is 631.73 Mb assembled from 576 contigs, with a contig N50 length of 5.36 Mb and scaffold N50 length of 61.15 Mb. Moreover, 99.83% (630.68 Mb) of ...
ChromosomeConformationCapture(3C)methodology.3Cconverts physicalchromatininteractionsintospecificligationproducts,whicharequantifiedindividuallybyPCR.Herewe presentahigh-throughput3Capproach,3C-CarbonCopy(5C),thatemploysmicroarraysorquantitativeDNA sequencingusing454-technologyasdetectionmethods.Weapplied5Ctoanalyzea400-kb...
Long-read DNA sequencing, optical mapping methods, and chromatin conformation capture techniques have been widely applied in whole-genome sequencing. These technologies are increasingly allowing chromosome-scale assemblies to be achieved [16]. At present, the nomenclature for newly assembled chromosomes is...
Chromosome conformation capture (3C) technology is a pioneering methodology that allows in vivo genomic organization to be explored at a scale encompassing a few tens to a few hundred kilobase-pairs. Understanding the folding of the genome at this scale is particularly important in mammals where di...