These high-GC primers also yielded increased numbers of amplification products in RAPDs on the DNAs of a broad range of other fungi.KubelikA. R.SzaboL. J.CURRENT GENETICSKubelik, A. R. & Szabo, L. J. (1995) High-GC primers are useful in RAPD analysis of fungi. Current Genetics 28...
3.高GC模板:在体系中加入终浓度为3%的DMSO可能有利于扩增。 4.不同模板的推荐使用量(25μL反应体系): 模板种类 扩增片段1 kb-10 kb 基因组DNA 50 ng-200 ng 质粒或病毒DNA 10 pg-20 ng cDNA 1-2.5 μL(不超过PCR反应总体积的1/10) PCR扩增程序:有以下三种程序可选择,优先选择两步法程序。
The Primer design criteria are pop- ulated in the textboxes within the "PRIMER DESIGN" frame (Fig. 2, circle 3). PrimerMapper will collect the primer design criteria defined by the user such as primer maximum and minimum lengths, GC%, and melting temperature (Tm), as well as the ...
which may give improved performance than only using the features automatically generated from the representation learning process. Motivated by this, we combined the secondary structural feature, GC content features, and thermodynamics features together with feature representations obtained...
Specific forward (5′- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG −3′) and reverse (5′- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′) primers targeting the 16S rRNA gene V3 V4 variable regions were used for amplification of the genomic template DNA as previously...
However, OCCC leads to a worse prognosis than HSGC due to OCCC chemoresistance to conventional platinum [11]. Therefore, exploring the cellular and molecular differences between OCCC and HGSC will help in developing alternative therapies. Single-cell RNA sequencing (scRNA-seq) has emerged as a ...
[23]. One of the drawbacks of TS is the difficulty of detecting clinically relevant variants, especiallyCNVs, in regions with an insufficient number of reads, leading to discontinuous coverage. This coverage lack may result from poor enrichment of GC-rich regions, and the absence of enrich...
PrimeScript II High Fidelity One Step RT-PCR Kit是在一个反应管中连续进行反应的1 step RT-PCR用试剂盒。反转录酶使用延伸性能好且能够合成全长cDNA的PrimeScript II RTase,PCR使用准确性高且适于扩增长链及GC Rich cDNA的PrimeSTAR®GXL DNA Polymerase,这两种酶经优化后可用于1 step RT-PCR。
It is very high fidelity polymerase even for long fragments like 12 kb and gc rich. already brought it!" —Shilpa Choudhury, GEORGIA INST OF TECHNOLOGY Find high-quality solutions for applications downstream of your PCR Looking for quick and dependable purification of your amplicon? Easilyclean up...
1)预变性温度和时间:推荐使用98℃。预变性推荐时间:质粒DNA等简单模板为30 sec;cDNA、基因组DNA等复杂模板为3 min;高GC含量模板为5-10 min。 2)退火温度和时间:推荐使用60℃,也可根据需要,设立温度梯度去摸索引物退火的最适温度。推荐退火时间设置为20 sec,可以在10-30 sec内调节。退火时间太长可能导致扩增产...