A standard curve may be used to determine the concentration of the target in your samples. However, determining the exact concentration is not required. In fact, a relative comparison between treated and control samples can be more informative than just the absolute concentration. Another reason...
Komatsu DE, Hadjiargyrou M (2004) Activation of the transcription factor HIF-1 and its target genes, VEGF, HO-1, iNOS, during fracture repair. Bone 34:680–688Komatsu DE, Hadjiargyrou M. Activation of the transcription factor HIF-1 and its target genes, VEGF, HO-1, iNOS, during ...
Insights into the role of the tumor suppressor pVHL in oxygen sensing motivated the testing of drugs that target the transcription factor HIF or HIF-responsive growth factors, such as VEGF, for the treatment of cancers caused by VHL inactivation, such as clear-cell renal cell carcinoma (ccRCC)...
3a), thus confirming that exposure of dams to chronic hypoxia truly activates the transcription of HIF target genes in the fetus. Next, we examined whole genome expression in fetal hypoxic kidneys using gene arrays. We identified 62 induced and 28 repressed genes compared to both control groups ...
B. The results of qPCR analysis demonstrate time-dependent changes of the expression of HIF target genes encoding PDH2, HK, LDHA, GLUT1 and PDK1. C. Quantitative analysis of the results shown in (A). N = 3, error bar show SD.
In our present study, we demonstrate that LOXL2 is a direct target of HIF-1 and that induction of LOXL2 is necessary and sufficient to repress E-cadherin under hypoxic conditions. HIF-1α plays an important role in the development of HCC by promoting HCC metastasis, EMT and VM through up...
The corresponding components and JGSQ for the intersection targets were screened, and a “JGSQ-component-target network” was constructed using Cytoscape 3.8.2. To investigate the protein‒protein interactions (PPIs) of JGSQ in treating DR, the drug-intersecting genes were uploaded to the STRING...
Hypoxia-inducible factors (HIFs) regulate gene transcription under conditions of low oxygen, and HIF target genes EPO and VEGF have been associated with muscle protection and repair. We tested the importance of HIF activation following skeletal muscle injury, in both a murine model and human ...
Recruitment of HIF-1α and HIF-2α to common target genes is differentially regulated in neuroblastoma: HIF-2α promotes an aggressive phenotype. Cancer Cell 10, 413-423.Holmquist-Mengelbier L, Fredlund E, Lofstedt T, Noguera R, Navarro S, Nilsson H, Pietras A, Vallon-Christersson J, ...
The effect of mTOR inhibition was slightly weaker in VHL-defective cells, probably reflecting a small negative effect of HIF1A on mTOR-target mRNAs, as outlined above. We also analyzed the effect of mTOR inhibition on the expression of genes involved in the HIF signaling pathway. This revealed...