Moreover, HGF treatment significantly downregulated the LPS-mediated induction of pro-inflammatory cytokines in primary cultured DRG neurons. Taken together, these data suggest that HGF-encoding plasmid DNA attenuates neuropathic pain via controlling the expression of pro-inflammatory cytokines....
To determine if HGF protects podocytes from HG-induced injury through inactivation of GSK3β, podocytes were transduced with adenovirus containing a wild-type GSK3β (Ad-wt), constitutively active GSK3β (Ad-S9A) or dominant-negative GSK3β (Ad-K85A) plasmid and were then subjected to HG ...
The Plasminogen-Apple-Nematode (PAN) domain, with a core of four to six cysteine residues, is found in > 28,000 proteins across 959 genera. Still, its role in protein function is not fully understood. The PAN domain was initially characterized in nume
Myocardial infarction (MI) is a leading cause of hospitalization worldwide. A recently developed strategy to improve the management of MI is based on the u
Methods: Four HGF variants (NK1, NK2, NK3, and NK4) were created by gene engineering, and the variant DNA fragments were cloned into pGEM-T for DNA sequencing and then transferred to a pTrcHis-A plasmid for expression. Recombinant pro- teins were purified from Escherichia coli, and a ...
Intracellular hydrogen peroxide levels were determined by a mammalian expression vector encoding a fluorescent hydrogen peroxide sensor pHyper (36). Cells were grown on glass bottom 35-mm dishes, transfected with 1 μg/ml of pHyPer-cyto plasmid, and treated as described above. Cells were washed tw...
NLS-GFP was inserted into a pME gateway vector. Final constructs were assembled in a pBHR4R3 plasmid (gift of the Brockerhoff lab) using the Gateway system (Life Technologies). Sparse transgenic labeling of cells was done by injecting one-cell-stage embryos with 50pg of plasmid and 50pg ...
(E) HepG2 cells were co-transfected with miR-198 mimics, plasmid reporter containing wild-type or mutant 3′UTR of c-MET. After 48 h, luciferase assay was performed and normalized to the internal firefly luciferase activity. ∗P < 0.01. 3.2. Forced expression of miR-198 reduces c-MET...
This was confirmed by data in Figure 2 as well, where administration of HGF/VEGF plasmid in HFD animals enlarged the AUC in GTT after surgery when compared to the state before the induction of ischemia. Thus, delivery of HGF/VEGF via GT may have an adaptive impact on systemic metabolism ...
Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease with no effective treatment. The Hepatocyte Growth Factor/Scatter Factor (HGF/SF), through its receptor MET, is one of the most potent survival-promoting factors for motor neurons (