Immunohistochemistry (IHC) is the most commonly used primary method for detection of HER-2 overexpression. Controversy exists on the interpretation of samples that are equivocal for HER 2 status (IHC 2+). Recent guidelines state that samples equivocal for HER 2 status require validation with ...
(20/71) of the FISH negative cases had chromosome 17 polyploidy.One IHC score(+++),FISH negative case and 7 IHC score(++),FISH negative cases were of chromosome 17 polyploidy.Conclusions A certain proportion of Her-2/neu IHC score negative cases may be positive when assessed by FISH,and...
Therefore, it seems misleading to state that 31.9% of noninvasive extramammary Paget's disease (EMPD) had overexpression of HER-2/neu, when the majority of these cases actually had equivocal staining (9 of the 15 cases diagnosed as positive were scored 2+, the other 6 cases scored 3+)....
Assessment of Her-2/ neu gene amplification status in breast carcinoma with equivocal 2+ Her-2/neu immunostaining. J Egypt Natl Canc Inst. 2011;23(1): 41-46.Mostafa NA, Eissa SS, Belal DM, Shoman SH. Assessment of Her-2/Neu gene amplification status in breast carcinoma with equivocal ...
Immunohistochemistry (IHC) is the most widely applicable diagnostic modality in studying HER-2 status. Fluorescence in situ hybridization (FISH) is also recognized as a modality in cases with an equivocal IHC status (score, 2+). Some authors claimed that FISH alone is sufficient. The aim of ...
On agarose CBs, 21/29 (72.4%) invasive breast carcinoma tumors were negative (0 or 1+) for HER-2/neu overexpression, 2/29 (6.9%) showed equivocal IHC staining (2+), and 6/29 (20.7%) tumors were positive (3+). IHC staining for HER-2/neu on agarose CBs showed 93.1% correlation ...
Neoadjuvant response pattern comparison between FISH proven Equivocal and IHC proven 3+ Her 2 neu receptor positive locally advanced breast cancer: a retrospective analysis from a prospectively maintained databasedoi:10.1016/S0960-9776(19)30278-4K. Goswami...