Immunohistochemistry (IHC) is the most commonly used primary method for detection of HER-2 overexpression. Controversy exists on the interpretation of samples that are equivocal for HER 2 status (IHC 2+). Recent guidelines state that samples equivocal for HER 2 status require validation with ...
ne the true HER2 status (Figs 1 and 2).54,55 The equivocal range for FISH assays is de?ned as HER2/CEP 17 ratios from 1.8 to 2.2 or average gene copy numbers between 4.0 and 6.0 for those systems without an internal control probe.34–36 Note, however, that patients with a HER2/...
HER2-heterogeneity(HER-2异质性)CAP Laboratory Improvement Programs Genetic Heterogeneity in HER2Testing in Breast Cancer Panel Summary and Guidelines Gail H.Vance,MD;Todd S.Barry,MD,PhD;Kenneth J.Bloom,MD;Patrick L.Fitzgibbons,MD;David G.Hicks,MD;Robert B.Jenkins,MD,PhD;Diane L.Persons,MD;...
Immunohistochemical analysis for Estrogen and Progesterone Receptor and Her-2/neu was done in all the cases. Fluorescent in situ hybridization for Her-2/neu gene amplification was performed in all 2+ cases and few equivocal 1+ and 3+cases by immunohistochemistry. Correlation betw...
Assessment of Her-2/ neu gene amplification status in breast carcinoma with equivocal 2+ Her-2/neu immunostaining. J Egypt Natl Canc Inst. 2011;23(1): 41-46.Mostafa NA, Eissa SS, Belal DM, Shoman SH. Assessment of Her-2/Neu gene amplification status in breast carcinoma with equivocal ...
P107 Predicting factor for HER-2 gene status in immunohistochemistry-equivocal (2+) breast cancerThis study investigated the associations between serum insulin-like growth factor-I (IGF-I) concentrations and erythropoietic activities in relation to body iron status. Serum IGF-I concentrations, free ...
HER-2proteinBackground In esophageal adenocarcinoma (EAC), concordance and prognostic significance of human epidermal growth factor 2 (HER-2) protein overexpression and gene amplification are equivocal, which led us to reevaluate this by immunohistochemistry (IHC) and in situ hybridization. Methods One...
Therefore, it seems misleading to state that 31.9% of noninvasive extramammary Paget's disease (EMPD) had overexpression of HER-2/neu, when the majority of these cases actually had equivocal staining (9 of the 15 cases diagnosed as positive were scored 2+, the other 6 cases scored 3+)....
HER-2 overexpression was evaluated by immunohistochemistry and in cases with equivocal expression, status was based on fluorescence in situ hybridization.Results:Over the study period, 56 cases of gastric and 42 cases of GEJ adenocarcinoma were identified for a total of 98 cases. The median age ...
Amplification of the HER2 gene was observed in 16% of equivocal to weakly positive cases. Those that were amplified showed low levels of amplification with ratios less than 4.5 and a characteristic scattered pattern of distribution of HER2 signals in the FISH assay. In addition, heterogeneity was...