Assessment of Her-2/ neu gene amplification status in breast carcinoma with equivocal 2+ Her-2/neu immunostaining. J Egypt Natl Canc Inst. 2011;23(1): 41-46.Mostafa NA, Eissa SS, Belal DM, Shoman SH. Assessment of Her-2/Neu gene amplification status in breast carcinoma with equivocal ...
ne the true HER2 status (Figs 1 and 2).54,55 The equivocal range for FISH assays is de?ned as HER2/CEP 17 ratios from 1.8 to 2.2 or average gene copy numbers between 4.0 and 6.0 for those systems without an internal control probe.34–36 Note, however, that patients with a HER2/...
HER2-heterogeneity(HER-2异质性)CAP Laboratory Improvement Programs Genetic Heterogeneity in HER2Testing in Breast Cancer Panel Summary and Guidelines Gail H.Vance,MD;Todd S.Barry,MD,PhD;Kenneth J.Bloom,MD;Patrick L.Fitzgibbons,MD;David G.Hicks,MD;Robert B.Jenkins,MD,PhD;Diane L.Persons,MD;...
Immunohistochemical analysis for Estrogen and Progesterone Receptor and Her-2/neu was done in all the cases. Fluorescent in situ hybridization for Her-2/neu gene amplification was performed in all 2+ cases and few equivocal 1+ and 3+cases by immunohistochemistry. Correlation betw...
P107 Predicting factor for HER-2 gene status in immunohistochemistry-equivocal (2+) breast cancerThis study investigated the associations between serum insulin-like growth factor-I (IGF-I) concentrations and erythropoietic activities in relation to body iron status. Serum IGF-I concentrations, free ...
'equivocal' and reflexively sent for FISH testing before rendering a result.3 Therefore, it seems misleading to state that 31.9% of noninvasive extramammary Paget's disease (EMPD) had overexpression of HER-2/neu, when the majority of these cases actually had equivocal staining (9 of the 15 ...
Therefore, it seems misleading to state that 31.9% of noninvasive extramammary Paget's disease (EMPD) had overexpression of HER-2/neu, when the majority of these cases actually had equivocal staining (9 of the 15 cases diagnosed as positive were scored 2+, the other 6 cases scored 3+)....
Her-2/neu immunodetection showed a 2+ equivocal positive rate, but Her-2/neu gene amplification was found on the cytological smear by fluorescence in situ hybridization analysis. Similar results were obtained within a tissue section. Concordant findings have been obtained when comparing the recent ...
HER-2proteinBackground In esophageal adenocarcinoma (EAC), concordance and prognostic significance of human epidermal growth factor 2 (HER-2) protein overexpression and gene amplification are equivocal, which led us to reevaluate this by immunohistochemistry (IHC) and in situ hybridization. Methods One...
Agreement varied within the different classes with highest agreement (up to 90%) for positive (3+) slides, and lowest agreement (72%-78%) for equivocal (2+) slides which contained ambiguous scoring. The developed automated method has the potential to be used as a computer aid for the ...