We have developed a new technique to quantify hepatitis C virus (HCV)-RNA using polymerase chain reaction (PCR) and dot blot hybridization. PCR was performed on 10-fold dilutions of an HCV-cDNA fragment for various numbers of cycles and on various serum volumes for 30 cycles. The resulting...
hepatitis C virus RNA, quantitative by TaqMan PCR 中文名: 中文别名: CBNumber: CB910079149 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 hepatitis C virus RNA, quantitative by TaqMan PCR化学性质 安全信息 hepatitis C virus RNA, quantitative by TaqMan PCR性质、用途与生产工艺...
The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients. 展开 关键词: qPCR Hepatitis B Hepatitis C
The quantification of hepatitis C virus (HCV) is essential for the management of chronic hepatitis C therapy. We have developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA in human EDTA-plasma and serum, and the performance of the method was assessed...
Method for qualitatively detecting high-risk HPV by using fluorescence quantitative PCR and kit thereof 本发明公开了一种荧光定量PCR定性检测高危型HPV的方法,该方法的荧光PCR反应体系中镁离子的浓度为1～10mM,Taq聚合酶0.1～5U,各种引物及探针浓度为100nM;在PCR反应前测... 张艳,李宁丽,顾关林,... - CN...
Quantitative real-time PCR (qRT-PCR) experiments were performed using primers listed in Table 1. qRT-PCR experiments were done using the CFX Connect real-time system (Bio-Rad Laboratories, Hercules, CA) under the following conditions: 15 min at 95 °C, followed by 40 cycles of 95 ...
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As indicators to determine the diagnosis, condition, and prognosis of hepatitis C, methods have been developed such as the detection of HCV-RNA by the nested RT-PCR method, quantitative assay of HCV-RNA by the competitive RT-PCR method, qualitative and quantitative assay of various anti-HCV Ig...
cells were all found to contain negative strands of HCV. Bone marrow cells (BMC) and oral tissues were also found to contain negative strands. For liver [25,26] and serum [25] the ratio of positive to negative strands were estimated to be between 1 and 1000 using semi-quantitative PCR....
Furtherly we extracted and sequenced the products, which help confirm the existence of C-Type rcDNA (Fig. S1A-B). Secondly, we conducted quantitative PCR to test the HBV DNA intermediates using specific primers [28] and the data showed that HepG2-17.3 produced comparable amounts of total ...