Nearly 60 million people worldwide are infected with Hepatitis C Virus (HCV), a bloodborne pathogen which leads to liver cirrhosis and increases the risk of hepatocellular carcinoma. Those with limited access to healthcare resources, such as injection dr
Results: A significant relationship between HCV RNA levels measured with the Xpert HCV Viral Load assay and the two commercial real-time PCR comparators (Abbott RealTime HCV test and Cobas AmpliPrep/Cobas Taqman HCV 2.0 test) was found in serum as well as in whole blood specimens. Conclusions...
which allowed for a faster run time (10—15 min) and lower operating temperature (35—420C) than LAMP. RPA works in a very similar way to RAA. The primary difference is the source of recombinase; RPA uses a bacteriophage
Real-time PCR targeting a 74-bp region of the HHV-6 DNA polymerase gene was performed using primers and probes previously described57 multiplexed with an internal positive control targeting mouse (mus) DNA spiked into each sample during DNA purification, as previously described58. In brief, each...
Real-time PCR has been performed as described in Fraga et al.55 using 2x TaqMan® Universal Master Mix II no UNG (Thermo Fischer) and a Quantstudio 5 apparatus (Thermo Fischer). Each 20-µL PCR reaction was performed with 5 µL of diluted cDNA. A standard curve prepared with dil...
The Cobas AmpliPrep/Cobas TaqMan HCV test, version 2.0, real-time PCR assay accurately quantifies hepatitis C virus genotype 4 RNA. The Cobas AmpliPrep/Cobas TaqMan HCV test,version 2.0,real-time PCR assay accurately quantifies hepatitis C virus genotype 4 RNA. Chevaliez S,Bouvier-... S ...
Real-time PCR was performed as outlined below. HBV DNA Quantification The level of HBV DNA in cell culture and liver tissue was measured by real-time PCR primers and probes listed in Table 2. Two primer pairs target segments in the S and C regions, and one pair a...
Quantitative real time PCR was performed using a Rotor Gene 3000 Termocycler (Corbett Research, Cambridge shire, UK) under the following conditions: 1 hold for 2 min at 50°C,1 hold for 5 min at 94°C, and 45 cycles of 30 sec at 94°C and 40 sec at 60°C. Specific primers for...
Quantitative real-time PCR (qRT-PCR) Total RNA from liver and intestinal tissues was isolated with TRIzol reagent and was reverse transcribed using the 5X All-In-One RT MasterMix kit (G490, Applied Biological Materials (ABM), Vancouver, BC, Canada) to yield the cDNA. A quantitative polymeras...
HBsAg quantification was done using Elecsys HBsAg Quant II (Roche Diagnostics, Indianapolis, USA) on a Roche Cobas e411 Immunoanalyzer following the manufacturer's protocol. 2.3. HBV DNA detection and analysis The HBV DNA level was determined from 500 μl of serum by quantitative real-time PCR ...