实时荧光定量PCR (Quantitative Real-time PCR)是一种在DNA扩增反应中,以荧光化学物质测每次聚合酶链式反应(PCR)循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。Real-timePCR是在PCR扩增过程中,通过荧光信号,对PCR进程进行实时检测。由于在PCR扩增的指数时期,模板的Ct值和该...
慢性乙型肝炎(轻度)患者血清验证荧光定量PCR法的特异性,并用不同浓度的质粒标准品检测该方 法的敏感性。结果证实HepG2.2.15细胞内存在cccDNA,其含量约为每个细胞18拷贝。对数生 长期前培养上清液和慢性乙型肝炎(轻度)患者血清均未检测到荧光信号,本实验条件下用该方法可 ...
Diagnosis of chronic hepatitis C infection is established by screening enzyme immunoassay to detect circulating antibodies to HCV proteins, followed by confirmatory determination of HCV RNA in serum, using qualitative and quantitative tests based on target (RT-PCR and transcription-mediated amplification) ...
Detection of micrometastasis in bone marrow in heptocellular carcinoma by real time quantitative RT-PCRrupture of the spleenacute pancreatitischronic pancreatitissplenectomypancreatectomyBackground: Accessory spleen is a common congenital anomaly. There are currently no endoscopic ultrasound (EUS) criteria ...
1C,D). The results of the cell cycle distribution and apoptosis analysis indicated that VCP2 delayed the cell cycle in G1 phase and induced apoptosis in HepG2 cells. Quantitative real-time PCR (qRT-PCR) and western blotting (WB) analysis...
Quantitative Reverse Transcription PCR (qRT-PCR). RNA was isolated from cells using RNeasy Mini Kit (Qiagen). Reverse transcription of RNA was performed with RT2 First Strand Kit (Qiagen) according to the manufacturer's protocol for usage of cDNA on Glycosylation RT2 Profiler PCR Array (...
Real time quantitative PCR (RT-PCR) confirmed the significant changes in 4 of 4 mRNAs assayed (CYP1A1, HO1, GSR, CDKN1), and identified 3 additional mRNA changes; 2 redox-related genes, gamma-glutamate-cysteine ligase modifier subunit (GCLM) and thioredoxin reductase (TXNRD1) and 1 DNA ...
为深入探讨蛋白酪氨酸磷酸酶-PEST在肝癌中的作用,构建了真核重组表达质粒pcDNA3.1/PTP-PEST并转染HepG2肝癌细胞,经荧光定量RT-PCR和Westernblot对转染细胞进行筛选,选出稳定高表达PTP-PEST基因的细胞株,并采用MTT及Westernblot对高表达PTP-PEST基因的细胞株的增殖速度和生长因子受体结合蛋白2表达量进行检测,观察到高表...
Quantitative PCR (qPCR) to measure abundance of expression of GLUTs and β-actin was performed with cDNA of HepG2 and human liver. Total RNA was extracted from HepG2 cells using the RNAeasy kit (Qiagen). Human liver total RNA was purchased (Clontech). Reverse-transcriptase (RT) reactions ...
Samples were then subjected to absolute real-time quantitative PCR (qPCR) using major capsid protein (MCP) primers to quantify TFV genomic concentration. Statistical analysis was performed using Student’s t-test (0.05 < *p ≤ 0.1, 0.01 < **p ≤ 0.05, 0.01 ≤ *...