Quantitative detection of hepatitis C virus RNA by multicyclic RT-PCR. Int Hepatol Commun 1993;1:72–79.Ishiyama N, Katayama K, Ishimi N, et al: Quantitative detection of hepatitis C virus RNA by multicyclic RT-PCR. Intern Hepatol Commun 1:72-79, 1993...
Quantitative PCR is the most sensitive test for determining hepatitis C viral load, whereas the branched-chain DNA test appears to be the most precise method. Major limitations of the current tests are inadequate dynamic range and high variability of PCR-based assays, and poor sensitivity of the...
The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients. 展开 关键词: qPCR Hepatitis B Hepatitis C
Method for qualitatively detecting high-risk HPV by using fluorescence quantitative PCR and kit thereof 本发明公开了一种荧光定量PCR定性检测高危型HPV的方法,该方法的荧光PCR反应体系中镁离子的浓度为1～10mM,Taq聚合酶0.1～5U,各种引物及探针浓度为100nM;在PCR反应前测... 张艳,李宁丽,顾关林,... - CN...
Zitzer, H.et al. Second-generation Cobas AmpliPrep/Cobas TaqMan HCV quantitative test for viral load monitoring: a novel dual-probe assay design., 571–577 (2013). CASGoogle Scholar Chevaliez, S., Bouvier-Alias, M. & Pawlotsky, J. M. Performance of the Abbott real-time PCR assay usin...
Furthermore, it is now ac(RT-PCR), i.e., RT-PCR techniques using synthetic internal cepted that quantitative HCV RNA tests will soon find a place control HCV RNA molecules as competitive standards for in the routine management of HCV-infected individuals. At quantification. In-house techniques...
hepatitis C virus RNA, quantitative by real-time PCR| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: hepatitis C virus RNA, quantitative by real-time PCR 英文同义词: hepatitis C virus RNA, quantitative by real-time PCR CAS号: 分子式: 分子量: 0 EINECS号: 信息错误报告 您的...
The most appropriate approach is to retest for anti-HCV using recombinant immunoblot assay (RIBA) and then test for HCV RNA using PCR assay in those who are RIBA positive or indeterminate. Liver histology is the gold standard in assessing severity of liver disease. Quantitative tests for serum ...
The COBAS AmpliPrep/COBAS Taqman HCV Quantitative Test, version 2.0, is an in vitro, fully automated, real-time, nucleic acid amplification test indicated for quantifying hepatitis C virus (HCV) RNA levels in the plasma/serum of patients infected with HCV of genotypes 1–6 who are receiving ...
Quantitative real-time PCR (qRT-PCR) experiments were performed using primers listed in Table 1. qRT-PCR experiments were done using the CFX Connect real-time system (Bio-Rad Laboratories, Hercules, CA) under the following conditions: 15 min at 95 °C, followed by 40 cycles of 95 ...