HCV RNA or viral load testing:If an antibody test is positive, the next step is to test for the actual virus to determine if a person still has active infection.Various methods are used to detect HCV RNA, including transcription-mediated amplification (TMR), polymerase chain reaction (PCR) a...
The UNG system in the commercial HCV-PCR test did not prevent five laboratories generating false-positive results in the 6 HCV-RNA negative samples. Among the laboratories with satisfactory results, up to 10000-fold differences in sensitivity were observed in the dilution series. The 50% and 90...
QIAGEN762431PAXgene Blood RNA MDx Kit (4) QIAGEN203745QIAGEN Fast Cycling PCR Kit (1000) QIAGEN203609HotStarTaq Plus DNA Polymerase (25000) QIAGEN201205Taq DNA Polymerase (1000) QIAGEN180850GeneRead qPCR FAST SYBR Green/ROX MM (2) QIAGEN159300QIAcard FTA Purification Reagent (500ml) ...
A,BRelative expression of lncRNA HEPFAL and SLC7A11 in 6 different liver cancer cell lines was detected by qRT-PCR.CThe expression of SLC7A11 in 6 different liver cancer cell lines was detected by western blot.D,E,G,HRelative expression of lncRNA HEPFAL and SLC7A11 in HCCLM3 and PL...
Dasabuvir is a nonnucleoside inhibitor of the hepatitis C virus (HCV) RNA-dependent RNA polymerase encoded by the gene for nonstructural protein 5B (NS5b), which is essential for replication of the viral genome; its antiviral activity is limited to HCV genotype 1. Ombitasvir is a NS5a ...
主营产品:PCR检测试剂盒、PCR试剂盒, 核酸检测试剂盒,荧光定量检测试剂.. 公司地址:上海市嘉定区嘉罗公路1661号 进入商铺收藏商铺 #动态#重组小鼠白介素-4(IL-4)的优势 #技术#人抗染色体抗体(anti-chromosomeAb)elisa定量检测试剂盒操作流程 组织来源:详见说明书 ...
慢性乙型肝炎(轻度)患者血清验证荧光定量PCR法的特异性,并用不同浓度的质粒标准品检测该方 法的敏感性。结果证实HepG2.2.15细胞内存在cccDNA,其含量约为每个细胞18拷贝。对数生 长期前培养上清液和慢性乙型肝炎(轻度)患者血清均未检测到荧光信号,本实验条件下用该方法可 ...
(Thermo Scientific). HepG2 cell lysate was incubated with 3 μg of purified biotinylated transcripts for 1 h at 25 °C, and then complexes were isolated with streptavidin-coupled T1 beads (Dynabeads, Thermo Scientific). The RNA present in the pulldown material was examined by RT-PCR...
Real-time qRT–PCR Total RNA was extracted from the HCC cells, and first-strand cDNA was synthesized using an oligo-dT primer and M-MLV reverse transcriptase (Invitrogen). Real-time qRT–PCR was performed using a Mx3000P QPCR System and MxPro Version 3.00 software (Stratagene, La Jolla, ...
RNA-Seq: RNA sequencing RT-PCR: Real-time polymerase chain reaction ddPCR: Droplet digital PCR RPKM: Reads per kilobase model per million mapped reads FDR: False discovery rate KEGG: Kyoto Encyclopedia of Genes and Genomes SRA: Sequence Read Archive ENCODE: The Encyclopedia of DNA El...