Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test. ...
Hepatitis C infection is diagnosed with several blood tests. The hepatitis C antibody test checks for antibodies (immune particles) that fight the virus. A "non-reactive" result means that antibodies to the virus are not detected. A "reactive" result means antibodies to the virus are present, ...
A test will be ordered to detect antibody to hepatitis A. The results of this test will also determine if the patient has been recently exposed to HAV. Blood probably will be tested for the hepatitis B and hepatitis Cviruses, as well as others others. For example, if a patient has had ...
Apo(a) in normal human serum can be detected at normal size with the same monoclonal antibody. Moreover, purchased monoclonal antibody to Lp(a) had been prepared to be highly specific to human apo(a) and to be not reactive to plasminogen. According to our results, it was suggested that ...
Subsequently, the slides were washed with 1× PBS (3 times for 3 min, at static condition and at room temperature), and incubated with secondary biotinylated goat anti-rabbit antibody for 1 h at static condition at 37 °C. The slides were then processed according to the manufacturer's ...
HepG2 cell lysate was prepared as described in the RNA pull-down assay section. Cell lysates from 1 × 106cells were immunoprecipitated using 2 μg eIF4A antibody or IgG at 4 °C overnight with rotation. The immunoprecipitates were digested with proteinase K (Thermo Scientific), an...
Compound Dilution Buffer, HCS Fixation Solution, Cell Permeabilization Buffer, Antibody Dilution Buffer and Wash Buffer should be stored at 2-8ºC. 20G needle/syringes and plate sealers may be stored at room temperature. All other components should be stored at -20ºC. Compound Dilution ...
The sections were washed with PBS 3 times for 3 min each, and a fluorescein isothiocyanate (FITC)-tagged secondary antibody (one drop/sheet, reaction at 37 °C for 30 min) was added dropwise; subsequently, the sections were washed with PBS 3 times for 3 min each. Then, the sections ...
Indeed, we recently revealed cell-specific differences between ARNT mRNA and protein expression in other cell lines.19 According to the general point of view ARNT is constitutively expressed.4 This means that neither ARNT mRNA nor protein level is affected by environmental conditions such as hypoxia...
Antibody of cyclin A2, Apf-1 and cytochrome C, p21, p27, and p53 were purchased from Cellsignaling (USA). The total RNA isolation kit and the two-step RT-PCR kit were purchased from Tiangen. All tumor cell lines were obtained from the Shanghai Institute for Biological Science (China)....