Host factors such as age, nutritional state, pregnancy, immunity from prior exposure, and co-infections all contribute to the outcome of virus infections. In natural infections of human populations it may be difficult to tease out the relative contribution of co-existing effects, but animal models...
Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production Nat. Genet., 26 (2000), pp. 435-439 View in ScopusGoogle Scholar [9] C. Bernal-Mizrachi, S. Weng, B. Li, L.A. Nolte, C. Feng, T. Coleman, J.O. Holloszy, C...
取100μl细胞悬液, 加入5μl Annexin V-FITC和10μl PI,混匀,室温避光反应15 min,PB洗涤1次,再加300斗1稀释的结合缓冲液,1 h内应用流式细胞术检测分析,每个样本收集1×104个荧光信号,采用Cell quest软件分析结果,实验重复3次。
以稀释的结合缓冲液(蒸馏水︰结合缓冲液 =1︰4),调整细胞密度为2×105~5×105个 /ml,取100μl细胞悬液, 加入5μl Annexin V-FITC和10μl PI,混匀,室温避光反应15 min,PB洗涤1次,再加300斗1稀释的结合缓冲液,1 h内应用流式细胞术检测分析,每个样本收集1×104个荧光信号,采用Cell quest软件分析结果,...