Rabbit anti-human thioredoxin polyclonal IgG1 (sc-20146) was from Santa Cruz Bio- technology (Santa Cruz, CA). All other chemicals were purchased from Sigma (St. Louis, MO). Cell culture and transfection Human embryonic kidney 293A (HEK293A) cells, purchased from the Cell Bank of the ...
与肝癌细胞系HepG2 特异性结合单链抗体的筛选与序列分析①
and then incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:1000) (Thermo Fisher Scientific) at room temperature for 1 h. Cells were resuspended in PBS and analyzed by flow cytometry (FACScan, BD Biosciences, San Jose, CA, USA). Data were calculated using Cell Quest Pro sof...
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