The repertoire of IgG antibody responses to infection and vaccination varies depending on the characteristics of the immunogen and the ability of the host to mount a protective immune response. Chronic hepatitis B virus (HBV) infections are marked by persistent infection and immune tolerance to vaccin...
VDJ gene segments were identified and aligned using IMGT/HighV-QUEST41. VDJ sequences were extracted to construct a personal immunoglobulin amino acid sequence library for MS/MS data- base search. Protein identification and quantification were performed using MaxQuant9. The intensities of iTRAQ ...
IgA, IgG, IgM Immunoglobulins A, G and M (respectively) κ-RF Kappa-Rheumatoid factor MGN Membranous glomerulonephritis MPGN Membranoproliferative (Mesangiocapillary) glomerulonephritis mTOR Mammalian target of rapamycin NHL Non-Hodgkin lymphoma NODAT New-onset diabetes after transplantation (Post-transplan...
To determine a patient’s HBV status, HBsAg is always tested alongside anti-HBc and anti-HBs (Fig.1). A positive HBsAg antigen indicates ongoing infection and infectivity. Initially, the majority of infections are hepatitis B e antigen (HBeAg)-positive. During the course of a chronic infection...
IgG-like (A–D) and non-IgG-like (E–J) bispecific antibody formats are depicted. (A) Duobody: its Fc region was suppressed by inserting mutations, which circumvents the Fc-mediated cytotoxicity. (B) Fc-modified IgG format: built with the knob into holes (KIH) technology to heterodimeriz...
To this extent, we sequenced IgG transcripts from 500,000 total B cells on the day of and 7 days following each vaccine to gain insight into total repertoire dynamics. Cell sorting was also performed to enrich for vaccine-specific cells and sequencing these enabled generation of a vaccine-...
The current invention contributes to the quest for candidate molecules for treatment and/or curing and/or prevention of HCV infection. As the candidate molecules are anti-HCV antibodies, they can also be applied for diagnosing HCV infection. Unexpectedly, the anti-HCV antibodies of the invention ar...
Antibodies cross-reactive to pathogens and autoantigens are considered pivotal in both infection control and accompanying autoimmunity. However, the pathogenic roles of autoantibodies largely remain elusive without a priori knowledge of disease-specific
to Model Hepatitis C Virus Treatment and Associated Resistance Ahmed Atef Mesalam 1,2, Koen Vercauteren 1,3 and Philip Meuleman 1,* 1 Center for Vaccinology, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent 9000, Belgium; ahmed.mesalam@ugent.be (A.A.M....