stem-/progenitor cells. Such quite high data may be explained by occasionally application of rhG-CSF therapy. Our results till now lead to the conclusion, that it seems to be useful, to recruit quantification of CD34+HPSC by additionally detecting CD133 antigens. This new stem cell marker (C...
We previously identified a distinct population of human circulating hematopoietic stem and progenitor cells (CHSPCs; CD14(-)glyA(-)CD34(+)AC133(+/-)CD45(dim)CD31(+) cells) in the peripheral blood (PB) and bone marrow, and their frequency in the PB can correlate with disease state. The...
Continuous supply of immune cells throughout life relies on the delicate balance in the hematopoietic stem cell (HSC) pool between long-term maintenance and meeting the demands of both normal blood production and unexpected stress conditions. Here we identified distinct subsets of human long-term (...
For example, SP-LSK cells are 100% CD34−/lowFlk-2−CD48−, and 90% with EPCR+ which can be further separated into CD150hi, CD150lo and CD150− sub-populations [53]. Thus, further combinations of these markers allowed for increased purity of HSCs [42, 43]. In addition, ...
cells (BMNCs), (2) CD34+cells (double column; CD34hi) enriched by immune-magnetic sorting (magnetic-activated cell sorting) and (3) flow-through (including CD34med/lowcells) labeled with anti-CD271 and a single-column selection enriched for both CD271+stroma and remaining CD34med/low-...
The consensus phenotype of primitive hematopoietic stem cells is CD34bright, CD38dim/-, HLA-DRdim/-, CD90+, CD117+, lineage−, and rhodamine 123lo.24, 25, 26, 27 In this report, we describe an immunization and screening procedure that has allowed the production of a novel MoAb, AC...
Specificity of CD64 for the granulo-monocytic lineage was shown by demonstrating that colonies arising from CD34+, CD64+ cells consisted of 98% ± 2% colony-forming unit-granulocyte-macrophage (CFU-GM) in semisolid medium containing stem cell factor (SCF), interleukin-3 (IL-3), IL-6, ...
We previously identified a distinct population of human circulating hematopoietic stem and progenitor cells (CHSPCs; CD14(-)glyA(-)CD34(+)AC133(+/-)CD45(dim)CD31(+) cells) in the peripheral blood (PB) and bone marrow, and their frequency in the PB can correlate with disease state. The...
Taking advantage of publicly available human databases, we observed that NOTCH3 mRNA is upregulated in HSPCs (CD133+CD34dim and CD38−CD34+) relative to most other blood compartments across the human hematopoietic system (Supplementary Fig. 1B) further supporting that NOTCH3 plays a function in...
CD133+CD34+VEGFR-3+ cells, as endothelial cell precursors, have dual functions as lymphatic endothelial progenitor cells and endothelial progenitor cells.66 VEGFR-3+ CD14+ monocytes in circulation also produce the lymphangiogenic cytokine VEGF-C, which may serve lympha- tic endothelial progenitor ...