Vasodilator-stimulated phosphoprotein (VASP) appears to be critical in cell-matrix adhesion and cell migration. Matrine, one of the major alkaloid components found in sophora roots, has long been regarded as an anticancer herb in China, which may inhibit the metastasis of cancer cells perhaps ...
We revealed that T286A, T288A, and T286A/T288A mutants were resistant to DIF-3-induced degradation compared with wild-type cyclin D1, indicating that the phosphorylation of Thr 286 and Thr 288 were critical for cyclin D1 degradation induced by DIF-3. Indeed, DIF-3 markedly elevated the...
Cells were lysed on ice in the lysis buffer (150 mm NaCl, 5 mm NaF, 2 mm Na3VO4, 50 mm Tris/HCl, pH 7.4, 1 mm EDTA, 1% (v/v) Tween 20, and 2 mm phenylmethylsulfonyl fluoride) and insoluble cell debris was removed by centrifugation at 5000 rpm for 3 min. The cell lysate ...
Fig. 1. Smac reduces the protein level of c-IAP1 and c-IAP2 in HeLa cells. HeLa cells were transfected with IAP alone (lanes 2, 4, and 6) or together with Smac (lanes 3, 5, and 7) or Smac alone (lane 1). Equal amounts of cell lysates (7 μg) were immunoblotted with the...
Cells mock-infected or infected with untreated IBV were used as controls. For cell treatments, V. cholerae neuraminidase at 10 mU was added to 6 )106 freshly digested HeLa cells in a 1.5-ml microfuge tube, and incubated at 378C for 1 h. The enzyme was removed by centrifugation, and the...
Cells were treated with hesperidin (0, 20, 40, 60, 80, and 100 μM) for 24, 48, or 72 h and relative cell viability was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results Hesperidin inhibited the proliferation of HeLa cells in a...
In infected cells, the chromatin structure of the adenovirus genome DNA plays critical roles in its genome functions. Previously, we reported that in early... T Komatsu,K Nagata - 《Journal of Virology》 被引量: 50发表: 2012年 Kinetics of inactivation of histone mRNA in the cytoplasm after ...
cells were imaged under continuous irradiation with 100% power of 488-nm laser embedded in the confocal system and an applied magnetic field varying between either + 10 mT and − 10 mT or + 20 mT and − 20 mT at frequencies of 0.1 Hz or 0.2 Hz. The spinning disk confocal...
The observed phenotypes in Nup88-overexpressing HeLa cells were not due to the progression of the epithelial-to-mesenchymal transition or activation of NF-κB, which are known to be important for cell migration and invasion. Instead, we identified an upregulation of matrix metalloproteinase-12 (...
The development of materials that allow proper functioning of cells on solid supports is directly relevant to the construction of living-cell biosensors. Both physical and chemical properties of the surfaces have been shown to be critical in this field. Our aim is to report correlations between ch...