1.Cells are maintained at 37±1℃in a humidified atmosphere containing 5%±1% carbon dioxide. Split the cells once they grow to 70-90% confluency. 2.Typically, when splitting confluent HEK 293T cells in a 1:5 ratio, confluency is reached again after 2-3 days; 1:10 ratio, 3-4 days;...
for the generation of universal cells Maryam Ranjbar1, Farshid Amiri1, Marjan Nourigorji1, Farid Torabizadeh2, Mahintaj Dara3 and Mehdi Dianatpour1,4* Abstract Background: Allogeneic stem cells are the most potent sources for replacing cell, tissue, and ...